在电压传感器中,一个封闭的电荷转移中心
Xiao Tao1, Alice Lee, Walrati Limapichat
1Laboratory of Molecular Neurobiology and Biophysics, Rockefeller University, Howard Hughes Medical Institute, 1230 York Avenue, New York, NY 10065, USA.
概括
电压传感器使用带电氨基酸来控制离子通道功能. 研究人员确定了一个电荷传输中心,可以精确操纵电压传感器运动和通道封锁.
科学领域:
- 生物物理学的生物物理.
- 分子生物学分子生物学
- 结构生物学 结构生物学
背景情况:
- 电压传感器对于电压依赖的离子通道和酶的功能至关重要.
- 它们对膜电压的快速反应依赖于电荷氨基酸 (氨酸或氨酸) 穿过电场的运动.
研究的目的:
- 在负责氨基酸运动的电压传感器内识别电荷转移中心.
- 调查特定突变如何影响对氨酸而不是氨酸的偏好.
- 了解如何操纵 lysine 放置可以稳定不同的电压传感器形状.
主要方法:
- 使用自然和非自然氨基酸的局部定向突变发生.
- 进行电生理学记录以评估通道功能.
- 采用X射线晶体学来确定高分辨率结构.
主要成果:
- 确定了一个电荷转移中心,包括一个刚性循环盖和两个与正电荷相互作用的负电荷.
- 证明特定的突变可以改变电荷转移中心对氨酸的偏好.
- 表明氨酸残留物的战略放置可以稳定特定的电压传感器构造.
结论:
- 识别的电荷转移中心是电压依赖的结构变化的关键.
- 素替代提供了一种精确控制电压传感器状态的方法.
- 这提供了一种强大的方法来剖析电压传感器机制及其与离子通道封锁的联系.
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相关概念视频
Voltage-gated Ion Channels
Voltage-gated ion channels are transmembrane proteins that open and close in response to changes in the membrane potential. They are present on the membranes of all electrically excitable cells such as neurons, heart, and muscle cells.
Generally, all voltage-gated ion channels have a 'voltage-sensing domain' that spans the lipid bilayer. The charged residues in the sensor move in response to the membrane potential changes that open the channel allowing ions movement. There are several types of...
Generally, all voltage-gated ion channels have a 'voltage-sensing domain' that spans the lipid bilayer. The charged residues in the sensor move in response to the membrane potential changes that open the channel allowing ions movement. There are several types of...
Voltage-gated Ion Channels
Voltage-gated ion channels are transmembrane proteins that open and close in response to changes in the membrane potential. They are present on the membranes of all electrically excitable cells such as neurons, heart, and muscle cells.
Generally, all voltage-gated ion channels have a 'voltage-sensing domain' that spans the lipid bilayer. The charged residues in the sensor move in response to the membrane potential changes that open the channel allowing ions movement. There are several types of...
Generally, all voltage-gated ion channels have a 'voltage-sensing domain' that spans the lipid bilayer. The charged residues in the sensor move in response to the membrane potential changes that open the channel allowing ions movement. There are several types of...
Non-gated Ion Channels
Ion channels are specialized proteins on the plasma membrane that allow charged ions to pass down their electrochemical gradient. Their main function is to maintain the membrane potential which is critical for cell viability. These channels are either gated or non-gated and can transport more than a thousand ions within milliseconds for the cellular event to occur.
Compared to the gated ion channels, the non-gated channels, also known as leakage or passive channels, have no gating mechanism.
Compared to the gated ion channels, the non-gated channels, also known as leakage or passive channels, have no gating mechanism.
Non-gated Ion Channels
Ion channels are specialized proteins on the plasma membrane that allow charged ions to pass down their electrochemical gradient. Their main function is to maintain the membrane potential which is critical for cell viability. These channels are either gated or non-gated and can transport more than a thousand ions within milliseconds for the cellular event to occur.
Compared to the gated ion channels, the non-gated channels, also known as leakage or passive channels, have no gating mechanism.
Compared to the gated ion channels, the non-gated channels, also known as leakage or passive channels, have no gating mechanism.
Mechanically-gated Ion Channels
Mechanically-gated ion channels are proteins found in eukaryotic and prokaryotic cell membranes that open in response to mechanical stress. Tension, compression, swelling, and shear stress can alter the conformation of the protein, opening a transmembrane channel that allows the passage of ions for signal transmission. In eukaryotes, mechanically-gated channels are distributed in several regions like the neurons, lungs, skin, bladder, and heart, where they play critical roles in numerous...
Mechanically-gated Ion Channels
Mechanically-gated ion channels are proteins found in eukaryotic and prokaryotic cell membranes that open in response to mechanical stress. Tension, compression, swelling, and shear stress can alter the conformation of the protein, opening a transmembrane channel that allows the passage of ions for signal transmission. In eukaryotes, mechanically-gated channels are distributed in several regions like the neurons, lungs, skin, bladder, and heart, where they play critical roles in numerous...
