通过时延显微镜对人类基因组的表型分析揭示了细胞分裂基因
Beate Neumann1, Thomas Walter, Jean-Karim Hériché
1MitoCheck Project Group, European Molecular Biology Laboratory (EMBL), Meyerhofstrasse 1, D-69117 Heidelberg, Germany.
Nature
|April 3, 2010
概括
研究人员使用一种新的高通量查平台确定了数百个重要的人类基因. 这一全基因组研究使我们对细胞分裂和迁移等基本生物过程的理解得到了进展.
科学领域:
- 基因组学就是基因组学.
- 细胞生物学 细胞生物学
- 生物信息学是一种生物信息学.
背景情况:
- 人类基因组是巨大的,但许多基因对于基本的生命功能至关重要,仍然没有特征.
- 了解基因功能对于破译复杂的生物过程至关重要.
研究的目的:
- 开发和实施一个高通量选平台,用于全基因组功能基因组学.
- 识别涉及基本细胞过程的新型人类基因.
主要方法:
- 利用RNA干扰 (RNAi) 进行强大的基因沉默.
- 采用时间间隔显微镜对光标记染色体的活细胞成像.
- 应用计算图像处理用于定量表型评分.
主要成果:
- 完成了大约21,000个人类蛋白质编码基因的全基因组表型分析.
- 确定了数百个参与细胞分裂,迁移和生存的基因.
- 作为Mitocheck联盟的一部分,生成了细胞分裂表型的全面数据集.
结论:
- 开发的平台可以进行高效,大规模的功能基因组学研究.
- 这项研究显著扩大了对人类细胞功能至关重要的基因目录.
- 高内容数据是公开的,为科学界提供了宝贵的资源.
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