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Updated: Jun 13, 2026

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Chromatin Isolation by RNA Purification (ChIRP)
Published on: March 25, 2012
长非编码RNAHOTAIR重编程染色质状态以促进癌症转移
Rajnish A Gupta1, Nilay Shah, Kevin C Wang
1Howard Hughes Medical Institute and Program in Epithelial Biology, California 94305, USA.
Nature
|April 16, 2010
概括
大型干预非编码RNAs (lincRNAs) 在乳腺癌中失调. 该lincRNAHOTAIR通过改变色素促进转移,建议它作为诊断和治疗的目标.
科学领域:
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 分子生物学分子生物学
- 在瘤学瘤学.
背景情况:
- 大量的干预非编码RNAs (lincRNAs) 被全基因组转录,但它们在人类疾病中的作用尚不清楚.
- 新出现的证据表明,lincRNAs与染色体重塑复合体相互作用.
- 对HOX loci lincRNAs的失调与癌症进展有关.
研究的目的:
- 研究lincRNAs,特别是HOTAIR在乳腺癌进展中的作用.
- 为了确定HOTAIR表达是否预测转移和患者存活率.
- 阐明HOTAIR影响癌细胞行为的分子机制.
主要方法:
- 在初级乳腺瘤和转移中对lincRNA表达的分析.
- 霍泰尔表达水平与临床结果的相关性.
- 功能性研究涉及强制性HOTAIR表达或癌细胞中的HOTAIR敲击.
- 染色体免疫沉测序 (ChIP-seq) 来评估Polycomb抑制复合体2 (PRC2) 的结合.
- 基因组甲基化分析 (H3K27me3) 和基因表达造型.
主要成果:
- 在乳腺瘤和转移中HOTAIR表达升高,与更差的预后相关.
- 在癌细胞中强制HOTAIR表达导致全基因组PRC2重新分配.
- 这种PRC2的再分配会改变基因组甲基化和基因表达,促进侵入性和转移.
- 霍泰尔的转移前作用取决于PRC2的活性.
- 霍泰尔损失抑制了侵入性,特别是在PRC2活性较高的细胞中.
结论:
- lincRNAs,以HOTAIR为例,在塑造癌症表观基因组方面发挥着积极的作用.
- 霍泰尔通过PRC2-介导的表观遗传修饰促进乳腺癌转移.
- 霍泰尔代表了乳腺癌转移的潜在生物标志物和治疗点.
相关概念视频
lncRNA - Long Non-coding RNAs
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...
lncRNA - Long Non-coding RNAs
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...
Types of RNA
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
RNA Performs Diverse...
Types of RNA
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
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Inheritance of Chromatin Structures
Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying DNA...
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Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
X-chromosome...