一个潜在的正反循环控制在酵母细胞周期开始时的CLN1和CLN2基因表达
1Rockefeller University, New York, New York 10021.
Cell
|May 31, 1991
概括
在酵母中,CLN1,CLN2和CLN3基因调节细胞循环. 它们的表达受到CDC28活动的控制,形成一个积极的反循环,对细胞周期的进展至关重要.
科学领域:
- 分子生物学分子生物学
- 酵母遗传学 酵母遗传学
- 细胞循环规则 细胞循环规则
背景情况:
- 在Saccharomyces cerevisiae中的CLN1,CLN2和CLN3基因是必不可少的和冗余的,在G1到S阶段过渡中发挥着关键作用.
- 众所周知,CLN1和CLN2mRNA水平受到交配费洛蒙和细胞循环进展出G1的负面调节.
- 由于CLN3mRNA表现出明显的监管控制,因此不受到与CLN1和CLN2相同的负面监管.
研究的目的:
- 研究控制Saccharomyces cerevisiae中CLN1和CLN2mRNA表达的调控机制.
- 阐明CLN基因功能,CDC28活性和细胞周期控制中的交配费洛蒙反应之间的相互作用.
- 为了确定CLN1和CLN2mRNA表达是否存在正反循环.
主要方法:
- 利用一个非功能性cln2基因与内部删除作为记者基因来评估mRNA表达.
- 研究了CLN3-2 (DAF1-1) 等位基因在防止细胞循环停止和mRNA转移中的作用.
- 检查了有效的cln2和cln1mRNA表达的活性CDC28和功能性CLN基因的要求.
主要成果:
- 证明CLN3-2等位基因抑制细胞循环停止和CLN1/CLN2mRNA下调作为对交配费洛蒙的反应,取决于活跃的CDC28.
- 在没有交配费洛蒙的情况下,有效的cln2mRNA表达需要活跃的CDC28和至少一个功能性的CLN基因.
- 对于来自非功能性cln1基因的mRNA观察到类似的调节模式.
结论:
- CLN基因功能和CDC28活动协同作用,增强CLN1和CLN2mRNA水平.
- 有证据表明,一种积极的反循环机制调节了CLN1和CLN2基因表达.
- 这些发现突出了酵母细胞周期进展所必不可少的协调调节网络.
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