周期性环林-Cdk活动引发了一个自主Cdc14释放振荡器
1The Rockefeller University, 1230 York Avenue, New York, New York 10065, USA.
Cell
|April 21, 2010
概括
一个控制Cdc14酸酶释放的新型细胞周期振荡器独立于循环林依赖激酶 (Cdk) 活动. 这种Cdc14振荡器是由Cdk活动驱动的,确保每个细胞周期释放一次.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 单核细胞分裂依赖于细胞周期的精确调节.
- 循环素依赖激酶 (Cdk) 活动振荡被认为是细胞周期进展的主要驱动因素.
- Cdk振荡和特定细胞周期事件之间的合机制仍然是积极研究的领域.
研究的目的:
- 研究芽酵母中Cdc14酸酶核子释放和再捕获的基础调节机制.
- 确定Cdc14释放振荡器与已确定的循环林依赖激酶 (Cdk) 振荡器之间的关系.
- 为了阐明这些振荡器是如何协调的,以确保适当的线粒 exit.
主要方法:
- 用了开花酵母作为模型生物.
- 采用活细胞成像技术来监测Cdc14酸酶局部化和动态.
- 操纵的环素-Cdk水平,以评估它们对Cdc14振荡器的影响.
- 分析了Cdc14释放的频率和时间,与Cdk活动相关.
主要成果:
- 确定了一种控制Cdc14酸酶释放和再封存的内在振荡模块.
- 证明这种Cdc14释放振荡器独立于恒定和生理周期-Cdk水平运行.
- 证明Cdc14振荡器的频率是由环林-Cdk活动调节的.
- 观察到Cdc14释放周期通过野生类型细胞中Cdk振荡器的引入与细胞周期同步.
结论:
- Cdc14释放振荡器是细胞循环控制网络中的一个自主模块.
- 通过Cdk振荡器对Cdc14振荡器进行训练,确保了线粒离子退出的精确时间.
- 这一发现表明了在真核细胞细胞循环调节中的振荡器合的更广泛原则.
- 这项研究为细胞循环控制系统的结构组织和进化动态提供了新的见解.
相关概念视频
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Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
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