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相关概念视频

Ligand Binding and Linkage00:49

Ligand Binding and Linkage

Allosteric proteins have more than one ligand binding site; the binding of a ligand to any of these sites influences the binding of ligands to the other sites. When a protein is allosteric, its binding sites are called coupled or linked.  In the case of enzymes, the site that binds to the substrate is known as the active site and the other site is known as the regulatory site. When a ligand binds to the regulatory site, this leads to conformational changes in the protein that can influence the...
Protein-protein Interfaces02:04

Protein-protein Interfaces

Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a polypeptide...

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相关实验视频

Updated: Jun 13, 2026

Development of a Backbone Cyclic Peptide Library as Potential Antiparasitic Therapeutics Using Microwave Irradiation
08:48

Development of a Backbone Cyclic Peptide Library as Potential Antiparasitic Therapeutics Using Microwave Irradiation

Published on: January 26, 2016

探索酶调节器的体空间.

Jinglin Fu1, Katherine Cai, Stephen Albert Johnston

  • 1Center for Single Molecule Biophysics, Arizona State University, Tempe, Arizona 85287, USA.

Journal of the American Chemical Society
|April 23, 2010
PubMed
概括
此摘要是机器生成的。

这项研究引入了一种用于选基阵列以寻找酶调节器的新方法. 该技术可以同时测量酶结合和活性,识别改变酶功能的.

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Peptide-based Identification of Functional Motifs and their Binding Partners
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Last Updated: Jun 13, 2026

Development of a Backbone Cyclic Peptide Library as Potential Antiparasitic Therapeutics Using Microwave Irradiation
08:48

Development of a Backbone Cyclic Peptide Library as Potential Antiparasitic Therapeutics Using Microwave Irradiation

Published on: January 26, 2016

Peptide-based Identification of Functional Motifs and their Binding Partners
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Peptide-based Identification of Functional Motifs and their Binding Partners

Published on: June 30, 2013

Computational Prediction of Amino Acid Preferences of Potentially Multispecific Peptide-Binding Domains Involved in Protein-Protein Interactions
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Computational Prediction of Amino Acid Preferences of Potentially Multispecific Peptide-Binding Domains Involved in Protein-Protein Interactions

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科学领域:

  • 生物化学 生物化学
  • 酶学 是一种酶学.
  • 化学生物学 化学生物学

背景情况:

  • 酶活性调节对于理解生物过程和开发疗法至关重要.
  • 需要高通量选方法来识别与酶功能相互作用并修改的分子.
  • 当前的方法在同时评估分子结合和功能影响时可能面临挑战.

研究的目的:

  • 开发和验证一种用于选高密度阵列的新方法,以发现与酶活性结合并调节的.
  • 为了使酶结合和活性在阵列上的单个点的同时测量.
  • 为了证明该方法在识别酶调节中的实用性.

主要方法:

  • 一种聚乙烯醇溶液应用于阵列表面,以限制产品在酶反应中的扩散.
  • 这允许在每个点同时测量酶活性和结合.
  • 该方法是使用大卜过氧化酶,性酸酶和β-galactosidase进行测试的.

主要成果:

  • 成功识别了与胡卜过氧化酶,性酸酶和β-galactosidase结合的.
  • 该方法证明了检测可显著改变这些酶活性的的能力.
  • 结合水平和酶活性进行了比较,以确定功能性结剂.

结论:

  • 这种方法为选高密度阵列以发现酶调节器提供了一个强大的平台.
  • 该技术允许同时评估绑定和功能调制,提高选效率.
  • 这种方法对于识别可改变酶活性的或小分子具有广泛的适用性.