通过microRNA165/6的细胞信号引导基因剂量依赖的根细胞命运
Annelie Carlsbecker1, Ji-Young Lee, Christina J Roberts
1Institute of Biotechnology/Department of Biosciences, University of Helsinki, FIN-00014, Finland.
Nature
|April 23, 2010
概括
植物根的发育依赖于细胞通信. 一个转录因子和微RNA在细胞之间移动,以剂量依赖的方式指导细胞模式,以适当的器官形成.
科学领域:
- 植物发育生物学植物发育生物学
- 分子植物科学 分子植物科学
- 植物器官生成 植物器官生成
背景情况:
- 植物根的辐射组织组织被保存.
- 血管组织的模式,如原氧体和甲氧体,对于器官发育至关重要.
- 这种模式背后的细胞间通信机制尚未完全理解.
研究的目的:
- 阐明植物根血管模式中细胞间通信的分子机制.
- 为了确定信号分子和途径,涉及到细胞类型的中心向导模式.
- 了解血管和内皮细胞之间如何交换位置信息.
主要方法:
- 研究了转录因子和微RNA的细胞间移动.
- 利用遗传分析研究基因激活和向mRNA降解.
- 研究了信号分子对细胞命运决定的剂量依赖作用.
主要成果:
- 血管圆柱体和内皮体之间的交叉声涉及SHORT ROOT (SHR) 和SCARECROW (SCR) 转录因子.
- 显示的SHR从血管气移动到内皮,激活SCR.
- 通过SHR/SCR识别了MIR165a和MIR166b微RNA的激活,然后向HD-ZIPmRNA,以剂量依赖的方式控制西细胞类型的特异性.
结论:
- 对体细胞模式的正信息是通过转录因子和microRNAs的方向运动传递的.
- 由转录因子调节的微RNA活性差异决定了西伦细胞类型的特异性.
- 这种信号网络确保了植物根血管组织的精确的辐射组织和功能.
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