收回:小RNA复合体作为植物细胞之间的移动静音信号起作用
Patrice Dunoyer1, Gregory Schott, Christophe Himber
1Institut de Biologie Moléculaire des Plantes du CNRS, Université de Strasbourg 12 rue du Général Zimmer, 67084 Strasbourg cedex, France. patrice.dunoyer@ibmp-ulp.u-strasbg.fr
概括
小干扰RNAs (siRNAs),而不是前体RNAs,在植物细胞之间移动以触发RNA沉默. 这种运动对于非细胞自主RNA干扰至关重要,可能涉及siRNA复杂体.
科学领域:
- 植物分子生物学 植物分子生物学
- 基因沉默机制 基因沉默机制
- 通过RNA干扰路径.
背景情况:
- 植物RNA干扰 (RNAi) 途径利用Dicer-like 4 (DCL4) 处理的小干扰RNA (siRNAs) 来通过Argonaute 1 (AGO1) 沉默目标RNA.
- 众所周知,植物中的RNAi是非细胞自主的,但移动信号的身份仍然难以捉摸.
研究的目的:
- 通过遗传学来确定植物RNA干扰通路中的移动静音信号的性质.
- 调查siRNA或其前体是否构成负责细胞间RNAi传播的移动信号.
主要方法:
- 利用DCL4功能的细胞特异性救援来评估siRNA的移动性.
- 采用细胞特异性抑制RNAi运动以阻止信号传输.
- 物理跟踪机械交付,标记siRNA复杂体,以观察它们的运动和功能.
主要成果:
- 确定外源性和内源性siRNAs,而不是前体分子,作为植物细胞之间的移动沉默信号起作用.
- 证明了标记siRNA复合体的物理转位,模仿了转基因RNAi的传播.
- 提供了证据表明,细胞对细胞的移动涉及siRNA复合体,而不是与AGO1.1结合的单链siRNA.
结论:
- siRNAs是植物中非细胞自主RNA干扰的移动信号.
- 移动沉默信号很可能以siRNA复杂体的形式存在,从而促进了全身基因沉默.
- 这一发现澄清了植物RNAi通路通信的一个基本方面.
相关概念视频
siRNA - Small Interfering RNAs
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
RNA Interference
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
RNA Interference
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
Experimental RNAi
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
Small interfering RNAs (siRNA)
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
piRNA - Piwi-interacting RNAs
PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...


