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Updated: Jul 18, 2026
08:48
Targeted in Situ Mutagenesis of Histone Genes in Budding Yeast
Published on: January 26, 2017
酵母组织素H4 N-终端序列是活体内促进体激活所必需的
L K Durrin1, R K Mann, P S Kayne
1Molecular Biology Institute, University of California, Los Angeles 90024.
Cell
|June 14, 1991
概括
基因素H4 N端对激活酵母中的特定基因促进体至关重要. 这个区域的修改显著影响基因转录调节.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 生物化学 生物化学
背景情况:
- 基因组是基本的蛋白质,它们将DNA包装成核体.
- 众所周知,组织蛋白的N端尾部经历了翻译后的修改,影响了基因表达.
- 了解基因组域在转录调节中的特定作用对于破译基因控制机制至关重要.
研究的目的:
- 在生物体内识别参与调节基因转录的基因组域.
- 为了研究基因组N端子,特别是基因组H4在促进体激活中的作用.
主要方法:
- 在Saccharomyces cerevisiae (酵母菌) 中,基因素的N末端产生了缺失和氨基酸替代.
- 评估了这些修改对GAL1和PHO5促进体激活的影响.
- 比较了对基因素H4和其他基因素 (H2A,H2B,H3) 修改的影响.
主要成果:
- 基因素H4 N-终端区域内的删除 (残留4-23) 显著降低了GAL1促进体激活 (20倍).
- 这种效应是特定于基因素H4的,因为H2A,H2B和H3N-终端的删除不会导致类似的减少.
- H4 N-终端删除也降低了PHO5促进体激活 (4至5倍).
- 在H4乙化位点和周围残留物的突变对促进体诱导产生了类似或更大的影响.
结论:
- 基因素H4的N端在激活特定基因促进体中起着关键作用,包括GAL1和PHO5.
- H4 N端可能与转录启动因子相互作用,促进核细胞的展开和基因表达.
- 基H4乙化位点及其周围的残留物是转录的重要调节元素.
相关概念视频
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The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...