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在小鼠染色体4上的一种特定菌株修饰剂控制着独立转基因基因位点的甲基化
P Engler1, D Haasch, C A Pinkert
1Department of Molecular Genetics and Cell Biology, University of Chicago.
Cell
|June 14, 1991
概括
鼠标菌株背景影响转基因甲基化. 一个单一的修饰基因 (Ssm-1) 控制了这种表观遗传特征,影响了淋巴组织中的V(D) J重组.
科学领域:
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 遗传学 是一个遗传学.
- 分子生物学分子生物学
背景情况:
- 转基因甲基化模式在老鼠菌株之间可以显著不同.
- 父母遗传可以影响初始的转基因甲基化水平.
研究的目的:
- 研究特定菌株转基因甲基化的遗传基础.
- 为了确定负责差异甲基化的修饰剂.
- 了解甲基化和V(D) J重组的关系.
主要方法:
- 交叉线 (C57BL/6和DBA/2) 的杂交,以研究转基因甲基化遗传.
- 使用BXD重组杂交的杂交小鼠和其他杂交菌株进行映射.
- 分析转基因甲基化模式和V(D) J在淋巴细胞组织中的重组.
主要成果:
- pHRD转基因表现出菌株依赖的甲基化,C57BL/6显示高甲基化,DBA/2显示低甲基化.
- 鉴定出一种与Fv-1不同的单一菌株特定修饰剂 (Ssm-1) 是导致甲基化差异的原因.
- 观察到父母对甲基化的影响,但随着反向交叉的继续而减少.
- 细胞马赛克主义导致一些小鼠的部分甲基化模式.
- pHRD转基因的V(D) J重组发生在淋巴细胞组织中的非甲基化副本中.
结论:
- 转基因甲基化是一种受特定遗传修饰剂影响的遗传表观遗传特征.
- Ssm-1 是转基因甲基化状态的一个关键决定因素.
- 转基因甲基化状态直接影响其对V(D) J重组的易感性.
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