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一种具有广泛基质范围的对抗选择性脂酶的定向进化,用于解α替代性

Karin Engström1, Jonas Nyhlén, Anders G Sandström

  • 1Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, SE-106 91 Stockholm, Sweden.

Journal of the American Chemical Society
|May 11, 2010
PubMed
概括

定向进化创造了一种Candida antarctica脂酶A (CalA) 变体,具有显著增强的活性和水解的广泛基质范围. 这种工程酶表现出反向的 (R) 选择性,提供了新的合成可能性.

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