相关实验视频
Updated: Jun 13, 2026

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Visualization of Replisome Encounters with an Antigen Tagged Blocking Lesion
Published on: July 27, 2021
通过复制因子C识别繁殖细胞核抗原的环开状态,促进了真核细胞片的加载
John A Tainer1, J Andrew McCammon, Ivaylo Ivanov
1Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, MB4, La Jolla, California 92037, USA.
Journal of the American Chemical Society
|May 12, 2010
概括
复制因子C (RFC) 与增殖细胞核抗原 (PCNA) 滑动结,稳定其开放的形状. 这种相互作用对于DNA复制和修复中的塞加载周期至关重要.
科学领域:
- 分子生物学分子生物学
- 生物化学 生物化学
- 结构生物学 结构生物学
背景情况:
- 增殖细胞核抗原 (PCNA) 是DNA复制,修改和修复所必需的关键滑动蛋白.
- 为了实现其功能,PCNA必须通过复制因子C (RFC) 加载器在原料模板连接处打开并重新密封.
- 了解RFC/PCNA相互作用机制是阐明过程性DNA复制的关键.
研究的目的:
- 在紧固件加载过程中调查RFC/PCNA复合体的结构变化.
- 阐明RFC在PCNA门打开和关闭机制中的作用.
- 在RFC的存在和缺失下分析PCNA形态转换的自由能量景观.
主要方法:
- 利用生物物理技术研究RFC/PCNA复合体的结构重组.
- 进行了PCNA开口的自由能量配置比较,有或没有RFC.
- 分析了RFC和PCNA的开放形态之间的交互接口.
主要成果:
- RFC绑定在RFC/PCNA复合体中引发了显著的结构重组,创建了一个扩展的接口.
- 在PCNA的开放状态下,与所有五个RFC子单元相连,经历了一个转变的自由能量景观,将其困在开放的构造中.
- RFC不会破坏PCNA的封闭状态;相反,它选择性地稳定了开放状态.
结论:
- 在塞加载周期中,RFC的主要作用是选择性稳定开放的PCNA形状.
- 交互机制强调了RFC在促进PCNA在DNA过程中的作用方面的功能.
- 这项研究为管理DNA复制忠实性和效率的分子机械提供了关键的见解.
相关概念视频
The Replisome
DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
S-Cdk Initiates DNA Replication
The cell cycle is a series of events leading to DNA duplication followed by the division of cell content to form two daughter cells. The cell cycle progresses in four stages—the cell increases in size (gap 1 or G1-phase), duplicates its DNA (synthesis or S-phase), prepares to divide (gap 2 or G2-phase), and divides (mitosis or M-phase).
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.
S-Cdk Initiates DNA Replication
The cell cycle is a series of events leading to DNA duplication followed by the division of cell content to form two daughter cells. The cell cycle progresses in four stages—the cell increases in size (gap 1 or G1-phase), duplicates its DNA (synthesis or S-phase), prepares to divide (gap 2 or G2-phase), and divides (mitosis or M-phase).
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.
Restarting Stalled Replication Forks
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart, a...
Positive Regulator Molecules
Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
Positive Regulator Molecules
To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.

