开发一种生物对等和高效的对量子点的结合方法,使用四素-诺伯烯循环添加
Hee-Sun Han1, Neal K Devaraj, Jungmin Lee
1Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139-4307, USA.
Journal of the American Chemical Society
|May 21, 2010
概括
我们开发了一种新的生物直角方法,用于将分子连接到量子点 (QD). 这种技术有效地标记癌细胞以用于向治疗.
科学领域:
- 生物结合化学 生物结合化学
- 量子点纳米技术 量子点纳米技术
- 生物对角化学 生物对角化学
背景情况:
- 量子点 (QD) 是多功能纳米材料,在生物成像和诊断领域具有应用.
- 有效和特定的结合方法对于开发有针对性的基于QD的theranostics至关重要.
- 现有的结合策略可能受到反应效率和特异性的限制.
研究的目的:
- 为量子点开发一种新的生物直角和模块化结合策略.
- 为了实现有机染料和生物分子与QDs的高效合.
- 为了证明这种方法在in situ癌细胞向中的实用性.
主要方法:
- 采用了诺波伦烯-四相反电子需求的迪尔斯-阿尔德循环加法反应.
- 采用非协调功能组来提高结合效率.
- 开发了norbornene功能化的QDs,用于随后的生物对等结合.
- 应用了以氨酸修饰蛋白标记的癌细胞在现场向的方法.
主要成果:
- 实现了高效的生物分子和有机染料对QDs的结合.
- 证明了norbornene-tetrazine循环添加的快速动力学.
- 成功地在现场针对诺波伦烯涂层QDs对活癌细胞进行了向.
- 在细胞环境中验证了生物对等反应的特异性.
结论:
- 本文所介绍的生物直角联方法为QD功能化提供了一个模块化和高效的方法.
- 这一策略能够精确地准癌细胞,为先进的治疗学应用铺平了道路.
- 诺波伦-四素的快速和特异性化学反应非常适合复杂的生物系统.
更多相关视频
11:02Genetic Encoding of a Non-Canonical Amino Acid for the Generation of Antibody-Drug Conjugates Through a Fast Bioorthogonal Reaction
Published on: September 14, 2018
10:54Preparation and Evaluation of 99mTc-labeled Tridentate Chelates for Pre-targeting Using Bioorthogonal Chemistry
Published on: February 4, 2017
相关概念视频
01:11Aryldiazonium Salts to Azo Dyes: Diazo Coupling
The reaction of weakly electrophilic aryldiazonium (also called arenediazonium) salts with highly activated aromatic compounds leads to the formation of products with an —N=N— link, called an azo linkage. This reaction, presented in Figure 1, is known as diazo coupling and occurs without the loss of the nitrogen atoms of the aryldiazonium salt. Highly activated aromatic compounds such as phenols or arylamines favor the diazo coupling reaction. The coupling generally occurs at the para position.
01:16Cycloaddition Reactions: Overview
Cycloadditions are one of the most valuable and effective synthesis routes to form cyclic compounds. These are concerted pericyclic reactions between two unsaturated compounds resulting in a cyclic product with two new σ bonds formed at the expense of π bonds. The [4 + 2] cycloaddition, known as the Diels–Alder reaction, is the most common. The other example is a [2 + 2] cycloaddition.