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Updated: Jun 12, 2026

Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering
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Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering

Published on: July 10, 2016

大分子的尺寸选择性分离通过纳米通道的titania膜,具有自清洁 (脱塞) 能力

Poulomi Roy1, Tuli Dey, Kiyoung Lee

  • 1Department of Materials Science, WW4-LKO, University of Erlangen-Nuremberg, Martensstrasse 7, 91058 Erlangen, Germany.

Journal of the American Chemical Society
|May 21, 2010
PubMed
概括

我们开发了一种简单的方法来制造可重复使用的二氧化纳米通道膜,用于分离蛋白质. 这些自清洁膜具有8-10nm通道,不需要热处理.

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Detergent Purification of Membrane Proteins

Detergents are used to purify the integral proteins of the membrane. The hydrophobic portion of the detergent can replace membrane phospholipids while solubilizing the membrane proteins. When detergent monomers reach a specific concentration in a solution called critical micelle concentration (CMC), they form micelles. Above CMC, the concentration of the detergent monomers remains in equilibrium with the micelle. The number of detergent monomers present in the CMC varies for each detergent, and...