控制Myxococcus发育的sRNA的适应性进化.
Yuen-Tsu N Yu1, Xi Yuan, Gregory J Velicer
1Department of Biology, Indiana University, Bloomington, IN 47405, USA.
Science (New York, N.Y.)
|May 22, 2010
概括
小RNAs (sRNAs) 调节了生物学. 一种特定的sRNA,Pxr,控制了Myxococcus xanthus的发育,其突变在作弊菌株中适应性恢复了发育.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 进化生物学 进化生物学
背景情况:
- 小RNA (sRNA) 分子是生物过程中的关键调节者.
- 在sRNA序列中适应进化的证据是有限的.
- Prokaryotes 中的多细胞发育涉及复杂的调节网络.
研究的目的:
- 确定和描述一个参与调节Myxococcus xanthus. xanthus多细胞发育的sRNA.
- 研究sRNA进化在表型适应中的作用.
- 证明sRNA调节器对进化变化的直接影响.
主要方法:
- 在Myxococcus xanthus.中识别了一种新型sRNA,Pxr.
- 对Pxr在调节果实体发育中的作用的功能分析.
- 基因操纵研究Pxr突变对M. xanthus作弊菌株的影响.
主要成果:
- 在M. xanthus.中,Pxr被确定为果实体发育的负调节者.
- 一个自发的Pxr突变取消了它的调节功能.
- 删除Pxr使得即使在营养丰富的条件下,也可以实现发育,绕过正常的饥饿触发器.
- 这种Pxr突变恢复了社会缺陷的M. xanthus cheater菌株的发育能力.
结论:
- Pxr 作为一个关键的检查点,控制了菌类细菌从生长到发育的过渡.
- 这项研究提供了直接的证据,即sRNA调节器可以驱动主要表型特征的适应性进化.
- sRNA分子控制 prokaryotes 中复杂的多细胞发育,促进进化适应.
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