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Updated: Jun 12, 2026

Measuring Cation Transport by Na,K- and H,K-ATPase in Xenopus Oocytes by Atomic Absorption Spectrophotometry: An Alternative to Radioisotope Assays
12:48

Measuring Cation Transport by Na,K- and H,K-ATPase in Xenopus Oocytes by Atomic Absorption Spectrophotometry: An Alternative to Radioisotope Assays

Published on: February 19, 2013

通过双电子-电子共振实验,通过Xenopus laevis卵细胞内的蛋白质的距离测定

Ryuji Igarashi1, Tomomi Sakai, Hideyuki Hara

  • 1Graduate School of Engineering, Kyoto University, Kyoto 615-8510, Japan.

Journal of the American Chemical Society
|June 2, 2010
PubMed
概括

双电子电子共振 (DEER) 成功分析了活细胞内的蛋白质结构. 这项技术测量了Xenopus laevis卵细胞中位点定向旋转标记的乌比奎在位点间的旋转距离.

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Purification and Reconstitution of TRPV1 for Spectroscopic Analysis

Published on: July 3, 2018

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...