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代和突变发生加速了酶立体选择性的实验室演变:与传统方法的严格比较

Manfred T Reetz1, Shreenath Prasad, José D Carballeira

  • 1Max-Planck-Institut für Kohlenforschung, Kaiser-Wilhelm-Platz 1, 45470 Mülheim an der Ruhr, Germany. reetz@mpi-muelheim.mpg.de

Journal of the American Chemical Society
|June 12, 2010
PubMed
概括

代和突变发生 (ISM) 显著增强了酶进化的立体选择性和稳定性. 这种方法被证明比以前的技术更有效,用于定向进化Pseudomonas aeruginosa lipase.

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