是人工RNA限制酶的PNA酶
Merita Murtola1, Malgorzata Wenska, Roger Strömberg
1Department of Biosciences and Nutrition, Karolinska Institutet, Novum, S-14183, Huddinge, Sweden.
Journal of the American Chemical Society
|June 16, 2010
概括
研究人员开发了新的PNAzymes,使用核酸的人工酶,作为特定序列RNases. 这些PNA酶有效地在精确的部位分裂RNA,模仿生物技术应用中的自然限制酶.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 合成化学 合成化学
背景情况:
- DNA限制酶是重要的研究工具,但缺少RNA分裂对应物.
- 现有的人工核糖核酶的裂变速率有限,需要固态度量.
研究的目的:
- 开发特定于位点和序列的RNA切割人工酶.
- 创建基于PNA的系统 (PNA酶),增强RNase活动和选择性.
主要方法:
- 设计和合成包含Cu (II) -2,9-二甲基南林组的PNA酶.
- 针对非基配对的RNA区域 (凸) 在PNAzyme结合时进行分裂.
- 评估分离率,基质周转率和序列歧视.
主要成果:
- 酶证明了特定于位点的RNA裂变,半衰期短至30分钟.
- 观察到有效的基质周转率,表明真实的酶行为.
- 随着序列的改变或不匹配,裂变率大幅下降,显示出高选择性.
结论:
- 酶作为有效的,序列特定的人造RNA酶而起作用.
- 这些系统表现出真正的酶特性,包括营业额和歧视.
- PNA酶为在体外应用中创建RNA限制酶提供了基础.
相关概念视频
Ribozymes
The term ribozyme is used for RNA that can act as an enzyme. Ribozymes are mainly found in selected viruses, bacteria, plant organelles, and lower eukaryotes. Ribozymes were first discovered in 1982 when Tom Cech’s laboratory observed Group I introns acting as enzymes. This was shortly followed by the discovery of another ribozyme, Ribonulcease P, by Sid Altman’s laboratory. Both Cech and Altman received the Nobel Prize in chemistry in 1989 for their work on ribozymes.
Ribozymes can be...
Ribozymes can be...
Ribozymes
The term ribozyme is used for RNA that can act as an enzyme. Ribozymes are mainly found in selected viruses, bacteria, plant organelles, and lower eukaryotes. Ribozymes were first discovered in 1982 when Tom Cech’s laboratory observed Group I introns acting as enzymes. This was shortly followed by the discovery of another ribozyme, Ribonulcease P, by Sid Altman’s laboratory. Both Cech and Altman received the Nobel Prize in chemistry in 1989 for their work on ribozymes.
Ribozymes can be...
Ribozymes can be...
Restriction Enzymes
Restriction enzymes are bacterial enzymes used to cut DNA in a sequence-specific manner. To cleave DNA, they bind to specific palindromic sequences called restriction sites. Such palindromic DNA sequences or inverted repeats are commonly found in regions of functional significance, such as the origin of replication, gene operator sites, and regions containing transcription termination signals.
The host bacteria protect their own genomic DNA from these enzymes by methylating these sites. Some...
The host bacteria protect their own genomic DNA from these enzymes by methylating these sites. Some...
RNA Interference
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
RNA Interference
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
Types of RNA
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...


