机器人创建了一个转录沉默的面
Michael S Y Huen1, Junjie Chen
1Genome Stability Research Laboratory, The University of Hong Kong, L1, Laboratory Block, 21 Sassoon Road, Hong Kong S.A.R.
Cell
|June 17, 2010
概括
ATM 激酶在DNA损伤反应中起着关键作用. 桑巴格和其他人Shanbhag等人. (2010) 发现ATM阻止了DNA双链断裂附近的转录,为DNA修复机制提供了新的见解.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 细胞生物学 细胞生物学
背景情况:
- 该ATM (ataxia-telangiectasia突变) 激酶是细胞对DNA损伤反应的关键调节者.
- 了解ATM在分子水平上的功能对于理解基因组稳定性至关重要.
研究的目的:
- 调查ATM激酶在调节DNA双链断裂附近的转录中的作用.
- 阐明ATM如何影响DNA损伤反应期间的基因表达动态.
主要方法:
- 在单细胞中同时监测转录和DNA损伤反应.
- 利用先进的显微镜和分子生物学技术,实时跟踪细胞事件.
主要成果:
- ATM激酶活性对于抑制DNA双链断裂部位附近的转录至关重要.
- 桑巴格和其他人Shanbhag等人. (2010) 证明了ATM在积极阻止损坏的染色质中转录的新功能.
结论:
- ATM 激酶在调节转录活性以应对DNA损伤方面具有以前未知的作用.
- 这一发现为DNA修复途径和基因表达调节之间的复杂相互作用提供了更深入的理解.
相关概念视频
Transcription Attenuation in Prokaryotes
Transcriptional attenuation occurs when RNA transcription is prematurely terminated due to the formation of a terminator mRNA hairpin structure. Bacteria use these hairpins to regulate the transcription process and control the synthesis of several amino acids including histidine, lysine, threonine, and phenylalanine. Transcription attenuation takes place in the non-coding regions of mRNA.
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
Bacterial Transcription
RNA polymerase (RNAP) carries out DNA-dependent RNA synthesis in both bacteria and eukaryotes. Bacteria do not have a membrane-bound nucleus. So, transcription and translation occur simultaneously, on the same DNA template.
Transcription can be divided into three main stages, each involving distinct DNA sequences to guide the polymerase. These are:
Transcription can be divided into three main stages, each involving distinct DNA sequences to guide the polymerase. These are:
Eukaryotic Transcription Inhibitors
Certain biochemical processes, such as embryonic development and cell growth regulation, depend on the repression of specific genes. DNA binding proteins known as eukaryotic transcription inhibitors regulate the repression of gene expression in eukaryotes. The presence of these inhibitors at the required location and time in the cell is triggered by the presence of hormones and additional signals from other cells.
Eukaryotic transcription inhibitors usually contain two distinct domains, a DNA...
Eukaryotic transcription inhibitors usually contain two distinct domains, a DNA...
Master Transcription Regulators
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
Master Transcription Regulators
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
Transcription
Transcription is the synthesis of RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in correctly synthesizing messenger RNA (mRNA). Transcriptional regulation is responsible for the differentiation of different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds of RNA Molecules
In eukaryotes,...
Transcription Can Produce Different Kinds of RNA Molecules
In eukaryotes,...


