在X染色体不活化过程中,在选择性异色染色体形成中的LINE-1活性
Jennifer C Chow1, Constance Ciaudo, Melissa J Fazzari
1Institut Curie, Paris F-75248, France.
Cell
|June 17, 2010
概括
长间隔元素 (LINEs) 在X染色体失活 (XCI) 中起着双重作用. 沉默的LINE有助于形成异色区,而活跃的LINE则有助于XCI扩散到容易逃逸的区域.
科学领域:
- 遗传学 是一个遗传学.
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 分子生物学分子生物学
背景情况:
- X染色体不活化 (XCI) 通过XistRNA使女性的一个X染色体沉默.
- XCI在染色体中传播的机制尚不清楚,其中涉及LINE-1元素.
研究的目的:
- 研究LINE在XCI扩散和异色染色体形成中的作用.
- 为了确定在XCI期间LINE表达是如何调节的.
主要方法:
- 在XCI期间对LINE元素表达和定位的分析.
- 调查LINE,异染色素和XistRNA之间的关系.
主要成果:
- 线路有助于形成XCI必不可少的无声核区.
- 在XCI期间表达出一组年轻LINE-1元素,这取决于Xist诱导的异色状态.
- 这些活跃的LINE位于易逃脱区域的XCI向基因附近,与内分RNA相关.
结论:
- 线路对XCI至关重要,在多个层面上发挥作用.
- 沉默的LINE有助于构建Xist诱导的异色区.
- 活跃的LINE促进了XCI在本地传播到容易逃逸的地区.
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Heterochromatin
The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at 9th...
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at 9th...
Heterochromatin
The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at 9th...
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at 9th...
Euchromatin
The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions take up more dye, appearing darker, while the less-compact areas take up less dye and appear lighter. Based on the compaction level, chromatins are classified into two primary forms – euchromatin and heterochromatin.
Euchromatin is the less dense region of the chromatin and stains lighter. Euchromatin contains histone H3 extensively...
Euchromatin is the less dense region of the chromatin and stains lighter. Euchromatin contains histone H3 extensively...
Euchromatin
The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions take up more dye, appearing darker, while the less-compact areas take up less dye and appear lighter. Based on the compaction level, chromatins are classified into two primary forms – euchromatin and heterochromatin.
Euchromatin is the less dense region of the chromatin and stains lighter. Euchromatin contains histone H3 extensively...
Euchromatin is the less dense region of the chromatin and stains lighter. Euchromatin contains histone H3 extensively...
Inheritance of Chromatin Structures
Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying DNA...
X-Inactivation
The human X chromosome contains over ten times the number of genes as in the Y chromosome. Since males have only one X chromosome, and females have two, one might expect females to produce twice as many of the proteins, with undesirable results.
