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相关概念视频

EDTA: Auxiliary Complexing Reagents01:26

EDTA: Auxiliary Complexing Reagents

EDTA titrations are usually carried out in highly basic conditions, where the fully deprotonated form of EDTA, Y4−, actively complexes with the free metal ions in the solution. Several metal ions precipitate as hydrous oxide (hydroxides, oxides, or oxyhydroxides) under these conditions, lowering the concentration of free metal ions in the solution. For this reason, auxiliary complexing agents or ligands such as ammonia, tartrate, citrate, or triethanolamine are used in EDTA titrations to...
EDTA: Chemistry and Properties01:22

EDTA: Chemistry and Properties

Polydentate ligands are most widely used in complexometric titrations because they form more stable complexes with the metal ions than mono- or bidentate ligands due to the chelate effect. Examples of polydentate ligands are ethylenediaminetetraacetic acid (EDTA), crown ethers, and cryptands. The most important feature of optimal polydentate ligands is the ability to form 1:1 complexes in a single-step process. Amino carboxylic acid derivatives are frequently used as complexing agents. EDTA is...
Preparation and Reactions of Thiols02:33

Preparation and Reactions of Thiols

Thiols are prepared using the hydrosulfide anion as a nucleophile in a nucleophilic substitution reaction with alkyl halides. For instance, bromobutane reacts with sodium hydrosulfide to give butanethiol.

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相关实验视频

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Electrochemical Preparation of Poly(3,4-Ethylenedioxythiophene) Layers on Gold Microelectrodes for Uric Acid-Sensing Applications
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Electrochemical Preparation of Poly(3,4-Ethylenedioxythiophene) Layers on Gold Microelectrodes for Uric Acid-Sensing Applications

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蛋白质结构敏感的电催化在dithiothreitol修饰的电极上

Veronika Ostatná1, Hana Cernocká, Emil Palecek

  • 1Institute of Biophysics, Academy of Sciences of the Czech Republic, v.v.i., Královopolská 135, 612 65 Brno, Czech Republic.

Journal of the American Chemical Society
|June 19, 2010
PubMed
概括

新的Dithiothreitol (DTT) 修改电极可以使用常流时电压计剥离 (CPS) 进行敏感蛋白质微分析. 这种方法将原生蛋白与变质蛋白区分开来,为生物医学和蛋白质组学应用提供了一个新的工具.

科学领域:

  • 电化学 电化学 电化学
  • 分析化学 分析化学
  • 生物化学 生物化学

背景情况:

  • 蛋白质分析在生物医学和蛋白质组学中至关重要.
  • 区分原生蛋白质和变质蛋白质是一项挑战.
  • 现有的方法在分析过程中可能无法保存蛋白质结构.

研究的目的:

  • 开发用于蛋白质微分析的新型Dithiothreitol (DTT) 改性电极.
  • 为了利用恒定电流的时间电流计剥离 (CPS) 进行敏感蛋白质检测.
  • 为了区分原生和变质蛋白质状态.

主要方法:

  • 发展Dithiothreitol (DTT) -水银和DTT-固体合金电极.
  • 在蛋白质检测中使用恒流时电流计剥离 (CPS).
  • 使用DTT修改的悬挂滴电极 (DTT-HMDE) 进行蛋白质分析.

主要成果:

  • 通过CPS峰值H (蛋白质催化进化) 检测到纳米分子度的蛋白质.
  • DTT自组装单层 (SAM) 保护蛋白质免受变质,同时允许电催化.
  • 牛血清白蛋白 (BSA) 和其他蛋白质的原生和变质形式是使用CPS峰值H进行区分的.

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Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange Mass Spectrometry for Studying Protein Structure and Dynamics
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Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange Mass Spectrometry for Studying Protein Structure and Dynamics

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Electrochemical Detection of Deuterium Kinetic Isotope Effect on Extracellular Electron Transport in Shewanella oneidensis MR-1
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Electrochemical Detection of Deuterium Kinetic Isotope Effect on Extracellular Electron Transport in Shewanella oneidensis MR-1

Published on: April 16, 2018

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Last Updated: Jun 12, 2026

Electrochemical Preparation of Poly(3,4-Ethylenedioxythiophene) Layers on Gold Microelectrodes for Uric Acid-Sensing Applications
10:48

Electrochemical Preparation of Poly(3,4-Ethylenedioxythiophene) Layers on Gold Microelectrodes for Uric Acid-Sensing Applications

Published on: July 28, 2021

Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange Mass Spectrometry for Studying Protein Structure and Dynamics
09:18

Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange Mass Spectrometry for Studying Protein Structure and Dynamics

Published on: April 17, 2017

Electrochemical Detection of Deuterium Kinetic Isotope Effect on Extracellular Electron Transport in Shewanella oneidensis MR-1
09:00

Electrochemical Detection of Deuterium Kinetic Isotope Effect on Extracellular Electron Transport in Shewanella oneidensis MR-1

Published on: April 16, 2018

  • 确定了特定的潜在区域,以保持本地蛋白质结构,变性或脱氧.

    • 结论:

    • 与CPS相结合的提改性电极为蛋白质分析提供了一个新的分析工具.
    • 这种方法可以区分蛋白质的结构状态.
    • 在电化学分析过程中,DTT SAM有效地保护蛋白质免受表面诱导的变性.