二甲胺生物合成需要由铁硫酶产生的有机基
Yang Zhang1, Xuling Zhu, Andrew T Torelli
1Department of Chemistry and Chemical Biology, Cornell University, Ithaca, New York 14853, USA.
Nature
|June 19, 2010
概括
对翻译延长因子2至关重要的二甲胺生物合成,通过识别新型铁硫酶Dph2.2来阐明. 这种酶独特地利用S-adenosyl-l-methionine产生激素,进步我们对这种基本修饰的理解.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 酶学 是一种酶学.
背景情况:
- 双胺是古生物和真核生物中翻译延长因子2的一个独特的翻译后修饰.
- 这种修改是喉毒素的目标.
- 在此之前,人们对涉及S-adenosyl-l-methionine (SAM) 的二甲胺基生物合成的初始步骤了解甚少.
研究的目的:
- 阐明二胺生物合成的第一步的机制.
- 为了识别和表征负责在archaeon Pyrococcus horikoshii.这个初始步骤的酶.
主要方法:
- 对Dph2酶的结构分析.
- 生物化学测试用于研究酶活性和机制.
- 研究铁硫集群在催化中的作用.
主要成果:
- 一种新型的铁硫聚合酶Dph2被认为是二甲胺生物合成的第一步的关键.
- Dph2是一种同聚体,每个单体结合一个 [4Fe-4S] 集群.
- Dph2通过从SAM中生成3-amino-3-carboxypropyl基来催化C-C键的形成,这与激进的SAM酶不同.
结论:
- Pyrococcus horikoshii Dph2代表了一种新的SAM依赖的,含有4Fe-4S的酶类.
- 该酶催化了二甲胺生物合成中前所未有的化学反应.
- 这一发现为翻译后修改的基本机制提供了关键的见解.
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