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可点击的NAD类似物用于标记poly ((ADP-ribose) 聚合酶的基底蛋白

Hong Jiang1, Jun Hyun Kim, Kristine M Frizzell

  • 1Department of Chemistry and Chemical Biology, Cornell University, Ithaca, New York 14850, USA.

Journal of the American Chemical Society
|June 22, 2010
PubMed
概括

研究人员开发了可点击的尼古丁胺胺氨基二核酸 (NAD) 类似物,以识别由Poly(ADP-ribose) 聚合酶 (PARP) 修饰的蛋白质. 这种方法成功标记和识别了79个潜在的PARP-1基质,进步了对细胞过程中PARP功能的理解.

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
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