增强的功能潜力核酸的aptamer图书馆图案增加二级结构的增强功能潜力
Karen M Ruff1, Thomas M Snyder, David R Liu
1Howard Hughes Medical Institute and the Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, Massachusetts 02138, USA.
Journal of the American Chemical Society
|June 23, 2010
概括
与标准库相比,设计用于增加二次结构的模式核酸库产生更高亲和度的aptamer. 这种方法增强了用于各种应用的功能核酸的发现.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 生物技术是生物技术.
背景情况:
- 在体外选择产生功能性核酸,如体和催化剂.
- 功能性核酸经常表现出重要的二次结构.
- 图书馆结构与阿普塔默产量之间的关系需要进一步研究.
研究的目的:
- 通过实验评估核酸库中增强的二次结构如何影响针对蛋白质点的aptamer生成.
- 为了比较模式图书馆 (R*Y*) 与标准随机图书馆 (N(60) 在aptamer选择中的有效性.
主要方法:
- 设计了一个有图案的核酸库 (R*Y*),以促进茎循环的形成.
- 计算预测了R*Y*库与N(60) 库的较高折叠能量.
- 使用两种库类型同时进行蛋白质结合的代体内选择.
主要成果:
- 在选择过程中,R*Y*图书馆在N(60) 图书馆上得到了持续的丰富.
- 三个目标中的两个最高亲和度的体源自R*Y*库.
- 在R*Y*图书馆中,识别的绑定动机的可能性明显更高.
结论:
- 有模式的核酸库增加了分离功能性体的可能性.
- 使用图案图书馆可以导致具有更好的结合亲和力的aptamers.
- 研究人员应该考虑图案图书馆,以更有效地发现aptamer和催化剂.
相关概念视频
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