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相关概念视频

Protein-Drug Binding: Determination Methods01:22

Protein-Drug Binding: Determination Methods

Determining protein-drug binding can be achieved through indirect and direct methods, each providing valuable insights into the interaction between proteins and drugs.
Indirect methods involve isolating the bound drug from its free form in biological samples such as blood, serum, or plasma. These techniques aim to measure the percentage of drugs bound to proteins. Equilibrium dialysis is a commonly used method where the free drug concentration at equilibrium is measured by separating the bound...

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相关实验视频

Updated: Jun 11, 2026

Identifying Protein-protein Interaction Sites Using Peptide Arrays
07:44

Identifying Protein-protein Interaction Sites Using Peptide Arrays

Published on: November 18, 2014

一种基于数组的方法来识别多价抑制剂.

Yalong Zhang1, Qian Li, Luis G Rodriguez

  • 1Chemical Biology Laboratory, National Cancer Institute, 376 Boyles Street, Building 376, Frederick, Maryland 21702, USA.

Journal of the American Chemical Society
|June 30, 2010
PubMed
概括
此摘要是机器生成的。

这项研究提出了一个新的甘氨酸阵列平台,通过不同的甘氨酸结构和呈现密度来分析碳水化合物-蛋白质相互作用. 该平台有效地识别了多价值探针和各种讲蛋白的抑制剂,这对于生物研究和治疗开发至关重要.

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Assessment of Resistance to Tyrosine Kinase Inhibitors by an Interrogation of Signal Transduction Pathways by Antibody Arrays
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Published on: September 19, 2018

Peptide-based Identification of Functional Motifs and their Binding Partners
14:28

Peptide-based Identification of Functional Motifs and their Binding Partners

Published on: June 30, 2013

相关实验视频

Last Updated: Jun 11, 2026

Identifying Protein-protein Interaction Sites Using Peptide Arrays
07:44

Identifying Protein-protein Interaction Sites Using Peptide Arrays

Published on: November 18, 2014

Assessment of Resistance to Tyrosine Kinase Inhibitors by an Interrogation of Signal Transduction Pathways by Antibody Arrays
07:42

Assessment of Resistance to Tyrosine Kinase Inhibitors by an Interrogation of Signal Transduction Pathways by Antibody Arrays

Published on: September 19, 2018

Peptide-based Identification of Functional Motifs and their Binding Partners
14:28

Peptide-based Identification of Functional Motifs and their Binding Partners

Published on: June 30, 2013

科学领域:

  • 生物化学 生物化学
  • 葡萄糖生物学 葡萄糖生物学
  • 化学生物学 化学生物学

背景情况:

  • 碳水化合物与蛋白质的相互作用在生物过程中至关重要.
  • 涉及连接体结构和呈现的多价值相互作用是紧密结合的关键.
  • 预测碳水化合物结合蛋白的最佳连接体呈现是具有挑战性的.

研究的目的:

  • 开发一个高通量甘氨酸阵列平台,用于分析碳水化合物-蛋白质相互作用.
  • 创建具有多样化的甘氨酸结构和呈现密度 (新甘氨酸蛋白密度) 的数组.
  • 为了确定多价值探针和各种lectins的抑制剂.

主要方法:

  • 开发了一个新的甘氨酸阵列平台,约有600种甘氨酸结构和呈现的组合.
  • 在数组表面上有不同的新甘氨蛋白密度,甘氨酸结构和甘氨酸密度.
  • 利用数组来区分不同类型的多价值复合体.

主要成果:

  • 成功测试了配列与植物讲解素:康卡纳瓦林A (conA),维基亚虫异质素B4 (VVL-B(4) 和Ricinus communis聚合素 (RCA120).
  • 快速识别出针对Pseudomonas aeruginosa lectin I (PA-IL) 和小鼠巨细胞银糖类型莱克 (mMGL-2) 的强效多价抑制剂.
  • 证明该平台能够识别抑制剂,而无需先前对目标讲素/受体的结构信息.

结论:

  • 开发的甘氨酸阵列平台对于碳水化合物-蛋白质相互作用的高通量分析是有效的.
  • 这种方法可以快速识别多价值探针和莱克的抑制剂.
  • 该平台为生物研究和开发针对碳水化合物-蛋白质相互作用的治疗剂提供了宝贵的工具.