在没有的情况下,LRRC26辅助蛋白允许在静止电压下激活BK通道
Jiusheng Yan1, Richard W Aldrich
1Section of Neurobiology, Center for Learning and Memory, University of Texas, Austin, Texas 78712, USA.
不易激发细胞中的大导电 (BK) 通道由LRRC26.2激活. 这种辅助蛋白能使BK通道在生理电压和水平下运行,而不依赖于膜去极化.
科学领域:
- 分子生物学分子生物学
- 细胞生理学 细胞生理学
- 离子通道功能的功能
背景情况:
- 大导电,电压和激活 (BK) 通道对于细胞刺激性至关重要.
- BK通道通常需要膜脱极化和高细胞的激活,这些条件在大多数不可激发细胞中没有得到满足.
- 辅助子单元在调节非刺激细胞中的BK通道关中的作用仍然不完全理解.
研究的目的:
- 为了研究BK通道激活在非激发性LNCaP前列腺癌细胞中的机制.
- 在非刺激细胞的生理条件下确定赋予BK通道活性的辅助蛋白.
- 通过辅助蛋白质阐明BK通道门调制的分子基础.
主要方法:
- 在LNCaP细胞中的电生理记录.
- 共同免疫沉用于识别相互作用的蛋白质.
- 位点定向突变发生以探测蛋白质相互作用和功能影响.
- 电压的分析,以确定BK通道封闭性质.
主要成果:
- 在LNCaP细胞中的BK通道可以在负膜潜力时被激活,而不会增加细胞质.
- 富含白的重复含有蛋白26 (LRRC26) 在这些细胞中与BK通道形成复合体.
- LRRC26显著地将BK通道激活的电压依赖性转移到更负的电位 (约. -140 mV) 的电压.
- LRRC26增强了电压传感器运动和通道打开之间的全联.
结论:
- LRRC26作为BK通道的新型辅助子单元,使其能够在不可激发的细胞中发挥作用.
- 该LRRC26-BK通道复合体允许在生理电压和水平下进行激活.
- 这一发现为非刺激性细胞类型中的BK通道活性提供了分子解释,影响了我们对细胞信号传递的理解.
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