相关实验视频
Updated: Jun 11, 2026
11:09
Chromatin Isolation by RNA Purification (ChIRP)
Published on: March 25, 2012
长非编码RNA作为基因组修饰复合物的模块化支架.
Miao-Chih Tsai1, Ohad Manor, Yue Wan
1Howard Hughes Medical Institute and Program in Epithelial Biology, Stanford University School of Medicine, Stanford, CA 94305, USA.
Science (New York, N.Y.)
|July 10, 2010
概括
长跨基因非编码RNAs (lincRNAs) 作为支架,结合不同的基因组修饰复合体. 这种RNA介导的组件通过指导特定的基因组修饰在染色质上来协调表观遗传.
科学领域:
- 分子生物学分子生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 基因组学就是基因组学.
背景情况:
- 长跨基因非编码RNAs (lincRNAs) 是染色质状态和表观遗传的关键调节者.
- 了解lincRNAs影响基因表达的机制对于破译表观遗传调节至关重要.
研究的目的:
- 为了研究lincRNAHOTAIR作为基因组修饰复合体的支架的作用.
- 阐明HOTAIR如何促进不同表观遗传修饰剂的组装和向染色质.
主要方法:
- 生物化学试验,以确定HOTAIR和基因组修饰复合体之间的结合相互作用.
- 染色体免疫沉 (ChIP) 评估复合体针对特定基因组位置的向性.
主要成果:
- 霍泰尔 (HOTAIR) 作为一个支架,通过其5'域绑定了多镇压复合体2 (PRC2) 和通过其3'域绑定了LSD1/CoREST/REST复合体.
- 通过HOTAIR通过RNA介导的PRC2和LSD1的组合使协调的素H3素27甲基化和素4脱甲基化成为可能.
- 这种协调的行动确定了质子修改对目标基因的模式.
结论:
- lincRNAs,以HOTAIR为例,可以作为组装基因组修饰酶的支架.
- 这种支架机制为RNA介导的染色质状态和表观遗传的调节提供了一个新的途径.
- 这些发现突出了lincRNA功能在指定表观遗传修饰方面的一般原则.
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The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...
Acetylation
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The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...
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01:23Types of RNA
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