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通过疫苗接种诱导广泛中和的H1N1流感抗体

Chih-Jen Wei¹, Jeffrey C Boyington, Patrick M McTamney

¹Vaccine Research Center, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Bethesda, MD 20892-3005, USA.

Science (New York, N.Y.)

|July 22, 2010

概括

使用血凝素 (HA) 的新型DNA prime/boost疫苗接种策略产生了针对多种H1N1流感菌株的广泛中和抗体,为通用流感疫苗提供了有前途的途径.

科学领域:

免疫学免疫学免疫学
疫苗学疫苗学疫苗学
病毒学病毒学

背景情况:

2009年流行性流感病毒突显了普遍流感疫苗的迫切需要.
现有的疫苗对多样化和快速演变的流感菌株提供有限的保护.

研究的目的:

开发一种疫苗接种策略,产生针对各种H1N1流感菌株的广泛中和抗体.
评估DNA prime/boost方法对通用流感疫苗开发的有效性.

主要方法:

用编码H1N1血质素 (HA) 的等离子体DNA接种动物的疫苗.
用季节性疫苗或编码HA的复制缺陷腺病毒5载体进行增强.
对历史和最近的H1N1菌株的抗体中和幅度的评估.
在小鼠和驼模型中评估对不同H1N1病毒的保护.

主要成果:

与单个组件相比,prime/boost策略显著提高了各种H1N1菌株 (1934-2007) 的中和率.
这种方法在小鼠和中提供了对不同H1N1病毒的保护.
引起的抗体向HA的保存干区域,并在非人类灵长类动物中观察到.

结论:

一种DNA原始/增强疫苗接种方法引起了对H1N1流感的广泛中和抗体.
这一战略表明,有可能开发一种对各种菌株有效的通用流感疫苗.
向保存的HA干区域是广泛流感保护的可行策略.

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