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外部脂质PI3P调解了真核病原体作用因子进入植物和动物宿主细胞的过程

Shiv D Kale¹, Biao Gu, Daniel G S Capelluto

¹Virginia Bioinformatics Institute, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA.

|July 27, 2010

概括

病原体效应蛋白使用一种保守的基因通过与酸-3-酸盐 (PI3P) 结合进入宿主细胞. 这种机制在植物,动物和人类细胞中广泛存在,提供了潜在的治疗点.

科学领域:

微生物学微生物学
细胞生物学细胞生物学
生物化学生物化学

背景情况:

病原体向宿主细胞输送效应蛋白,以解除宿主防御的作用.
菌体利用N端RXLR和dEER的动机使效应体进入宿主细胞.

研究的目的:

研究RXLR图案在真菌效应器中的作用.
为了确定效应器进入植物和动物细胞的机制.
探索PI3P作为治疗干预的目标.

主要方法:

对RXLR动图变异的真菌效应因子的序列分析.
生物化学试验以评估效应因子与酸-3-酸盐 (PI3P) 的结合.
使用植物,动物和人类细胞进行细胞培养实验,以观察效应器的进入.
通过阻断PI3P结合来进行抑制测定,以研究其在内细胞分裂中的作用.

主要成果:

效应器包含RXLR图案的功能变体.
菌体和真菌RXLR基因都与PI3P结合.
PI3P存在于植物和动物细胞的血膜上.
作用因子通过脂质介导的内细胞突变进入植物,动物和人类细胞.
阻止PI3P结合会抑制效应器的进入.

结论:

RXLR基因和PI3P结合是对各种病原体进行效应器进入的保存机制.
通过PI3P介导的效应因子进入是植物,动物和人类病原发生的一个广泛现象.
准PI3P结合是一种有前途的治疗策略,可以对抗病原体感染.

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