通过调节L型Ca2+) 通道,Wnt11模拟了心肌电梯度
Daniela Panáková1, Andreas A Werdich, Calum A Macrae
1Brigham and Women's Hospital/Harvard Medical School, Cardiovascular Division, 75 Francis Street, Thorn 11, Boston, Massachusetts 02115, USA.
Nature
|July 27, 2010
概括
电梯度对于生物发育至关重要. 这项研究揭示了非正规的Wnt11信号通过调节斑马鱼心中的L型通道来建立心肌电梯度.
科学领域:
- 发展生物学 发展生物学
- 电子生理学 电子生理学
- 细胞信号传递 细胞信号传递
背景情况:
- 电梯度对于生物过程如器官生成和伤口愈合至关重要.
- 调节电极性建立和维护的机制尚不清楚.
- 了解这些机制是解读基本发育过程的关键.
研究的目的:
- 确定在发育中的心肌中建立电梯度的基础机制.
- 调查Wnt11信号在心脏电极性中的作用.
- 阐明参与产生心肌电梯度的分子参与者.
主要方法:
- 高速光学绘制斑马鱼心中的跨膜潜力和度.
- 研究了细胞刺激性,连xin定位,组织几何和机械输入的作用.
- 利用基因操纵来评估Wnt11信号通路.
主要成果:
- 识别了一种电气合的梯度,穿过正在发育的心室肌肉.
- 证明了这种心肌电梯度需要非正规的Wnt11信号.
- 显示的Wnt11通过L型通道调节跨膜Ca(2+) 导电,独立于平面细胞极性通路.
结论:
- 非正规的Wnt11信号通过L型通道介导的Ca2+) 导电率建立心肌电梯度.
- 这揭示了Wnt/Ca2+) 信号在心脏发育和电极性的新角色.
- 通过Wnt信号调节细胞合可能是发展中的一般机制.
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