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相关实验视频

Updated: Jun 10, 2026

Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level
08:29

Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level

Published on: April 19, 2019

在单细胞中量化具有单分子敏感性的大肠杆菌蛋白质组和转录组.

Yuichi Taniguchi1, Paul J Choi, Gene-Wei Li

  • 1Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA.

Science (New York, N.Y.)
|July 31, 2010
PubMed
概括
此摘要是机器生成的。

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单个细菌细胞在蛋白质和信使RNA (mRNA) 水平上表现出细胞间的变化. 这些分子数在任何特定基因的蛋白质和mRNA之间没有相关性.

科学领域:

  • 细菌的基因表达方式
  • 单细胞生物学 单细胞生物学
  • 分子生物学分子生物学

背景情况:

  • 在细菌群体中观察到蛋白质和信使RNA (mRNA) 副本数的细胞间变异性.
  • 低分子数使得单细胞中检测具有挑战性.

研究的目的:

  • 在单个细菌细胞中对蛋白质和mRNA表达进行定量系统范围的分析.
  • 在分析基因表达方面实现单分子灵敏度.

主要方法:

  • 利用新建的黄色光蛋白融合库用于大肠杆菌.
  • 对蛋白质和mRNA表达进行了系统范围的定量分析.
  • 应用单分子灵敏度检测方法.

主要成果:

  • 单细胞中的蛋白质数量分布在很大程度上遵循马分布.
  • 在低表达下,分布参数与转录速率和蛋白质突发大小有关.
  • 外在噪声显著影响在高表达水平的分布.
  • 给定基因的蛋白质和mRNA拷贝数在单个细胞内是不相关的.

结论:

  • 马分布有效地模拟了单个细菌细胞中的蛋白质数量变化.

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Probing mRNA Kinetics in Space and Time in Escherichia coli using Two-Color Single-Molecule Fluorescence In Situ Hybridization
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Method for Labeling Transcripts in Individual Escherichia coli Cells for Single-molecule Fluorescence In Situ Hybridization Experiments
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Method for Labeling Transcripts in Individual Escherichia coli Cells for Single-molecule Fluorescence In Situ Hybridization Experiments

Published on: December 21, 2017

相关实验视频

Last Updated: Jun 10, 2026

Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level
08:29

Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level

Published on: April 19, 2019

Probing mRNA Kinetics in Space and Time in Escherichia coli using Two-Color Single-Molecule Fluorescence In Situ Hybridization
10:01

Probing mRNA Kinetics in Space and Time in Escherichia coli using Two-Color Single-Molecule Fluorescence In Situ Hybridization

Published on: July 30, 2020

Method for Labeling Transcripts in Individual Escherichia coli Cells for Single-molecule Fluorescence In Situ Hybridization Experiments
07:51

Method for Labeling Transcripts in Individual Escherichia coli Cells for Single-molecule Fluorescence In Situ Hybridization Experiments

Published on: December 21, 2017

  • 区分内在和外在噪声对基因表达变异性的贡献.
  • 突出了单个细菌细胞中mRNA和蛋白质水平之间缺乏相关性.