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相关概念视频

Nitric Oxide Signaling Pathway01:28

Nitric Oxide Signaling Pathway

Nitric oxide (NO), an inorganic gas, acts as a potent second messenger in most animal and plant tissues. NO diffuses out of the cells that produce it and enters the neighboring cells to generate a downstream response. NO synthase (NOS) catalyzes NO production by the deamination of the amino acid arginine. There are three isoforms of NOS. Endothelial cells have endothelial NOS (eNOS), nerve and muscle cells have neuronal NOS (nNOS), and macrophages produce inducible NOS (iNOS) upon exposure to...

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相关实验视频

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Application of Genetically Encoded Fluorescent Nitric Oxide (NO&#8226;) Probes, the geNOps, for Real-time Imaging of NO&#8226; Signals in Single Cells
08:32

Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells

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一种高度选择性的低背景光成像剂,用于氧化的氧化.

Youjun Yang1, Stephanie K Seidlits, Michelle M Adams

  • 1Department of Chemistry and Biochemistry, University of Texas at Austin, Austin, Texas 78712, USA.

Journal of the American Chemical Society
|August 3, 2010
PubMed
概括

研究人员开发了NO(550),这是一种用于检测氧化 (NO) 的新型探针. 这种易于合成的化合物为细胞内和细胞外NO量化提供了快速,选择性和敏感的方法.

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科学领域:

  • 化学传感器是一种化学传感器.
  • 生物医学成像学 生物医学成像学
  • 分子探测器分子探测器

背景情况:

  • 氧化 (NO) 是一个关键的信号分子.
  • 现有的NO检测方法在选择性和灵敏性方面面临挑战.
  • 开发先进的探测器对于生物研究至关重要.

研究的目的:

  • 引入一种用于检测氧化 (NO) 的新型传感机制.
  • 开发一种易于合成和高效的NO探头.
  • 评估探头在细胞内和细胞外NO量化方面的性能.

主要方法:

  • 一个新的NO探头的合成,指定NO(550).
  • 探测器对NO的反应的表征,包括信号启动和线性.
  • 对干扰物种的选择性评估和细胞膜透性的评估.
  • 在体外研究使用两个细胞系来确定细胞相容性.

主要成果:

  • NO(550) 呈现出快速,线性和红移1500倍的启动信号,用于NO检测.
  • 探测器对反应性氧/物种,pH和常见干扰物的选择性非常出色.
  • NO ((550) 能有效地穿过细胞膜,但不能穿过核膜,从而可以进行细胞内和细胞外NO测量.
  • 在体外研究证实了与测试细胞系的良好细胞相容性.

结论:

  • NO(550) 是一种卓越的探测器,因为它具有高特异性,深色背景,易于合成和低pH依赖性.
  • 探测器的特性使其非常适合在生物系统中用于NO量化和成像应用.
  • 这一发展为研究人员研究NO在各种生理和病理过程中的作用提供了宝贵的工具.