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黄金纳米粒子丰富方法用于识别蛋白质中的S-化和S-化位

Adam Faccenda1, Christopher A Bonham, Panayiotis O Vacratsis

  • 1Department of Chemistry & Biochemistry, University of Windsor, 401 Sunset Avenue, Windsor, Ontario, Canada.

Journal of the American Chemical Society
|August 4, 2010
PubMed
概括

黄金纳米粒子 (AuNPs) 提供了一种简单的方法来分离和丰富醇修饰,用于识别蛋白质S修饰位. 这种技术有助于分配易受伤害的醇,从而推进蛋白质组分析.

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Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.