基于石墨烯的SELDI探针具有超高的提取能力和对DNA寡合物的敏感性
Lena Ai Ling Tang1, Junzhong Wang, Kian Ping Loh
1Department of Chemistry, National University of Singapore, 3 Science Drive 3, 117543, Singapore.
Journal of the American Chemical Society
|August 12, 2010
概括
石墨烯作为一个高效的平台,用于集中和检测单链DNA (ssDNA). 这种方法允许使用SELDI-TOF-MS (表面增强激光吸附离子-飞行质量谱的时间) 进行直接的,无标签的分析.
科学领域:
- 材料科学 材料科学 材料科学
- 生物技术是生物技术.
- 分析化学 分析化学
背景情况:
- 石墨烯的独特特性为先进的生物传感应用提供了潜力.
- 有效的核酸预度和检测对于基因组和蛋白质组分析至关重要.
研究的目的:
- 为了证明石墨烯作为SSDNA预度和检测的超高效率平台的实用性.
- 在SELDI-TOF-MS中探索DNA吸附石墨烯的直接应用.
主要方法:
- 使用石墨烯作为SSDNA吸附的基质.
- 使用表面增强激光吸附离子化-飞行质谱时间 (SELDI-TOF-MS) 直接分析ssDNA吸附的石墨烯.
主要成果:
- 石墨烯使ssDNA的超高效率预缩成为可能.
- 通过使用SELDI-TOF-MS.成功地在石墨烯上直接,无标签检测ssDNA.
结论:
- 石墨烯是一种可行的,高效的SSDNA分析平台.
- 开发的方法显示出在蛋白质组学和基因组学中进行快速,无标签分析的潜力.
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