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Efficient Construction of Drug-like Bispirocyclic Scaffolds Via Organocatalytic Cycloadditions of α-Imino γ-Lactones and Alkylidene Pyrazolones
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聚化乙和9-epi-聚化乙的总合成

K C Nicolaou1, Jianhua Wang, Yefeng Tang

  • 1Department of Chemistry and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA. kcn@scripps.edu

Journal of the American Chemical Society
|August 12, 2010
PubMed
概括

研究人员实现了独特的二次代谢物 sporolide B. 的总合成. 这是通过开发新的分子内部异质迪尔斯-阿尔德反应和催化循环添加物而实现的.

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Industrial insulin production uses genetically engineered E. coli expressing a proinsulin gene controlled by a tryptophan promoter and containing a methionine linker for later cleavage. The cells also carry ampicillin resistance for selective growth. Seed cultures are stored at −80 °C and production begins by thawing a small amount to inoculate starter cultures, which are progressively scaled to a 50,000-L bioreactor. In the bioreactor, E. coli grow in nutrient-rich media under sterile, tightly...