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相关概念视频

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...

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相关实验视频

Updated: Jun 10, 2026

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
08:40

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Published on: April 6, 2012

哺乳动物的微RNA主要作用于降低目标mRNA水平.

Huili Guo1, Nicholas T Ingolia, Jonathan S Weissman

  • 1Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, USA.

Nature
|August 13, 2010
PubMed
概括
此摘要是机器生成的。

微RNAs (miRNAs) 主要通过降低目标信使RNA (mRNA) 水平来降低蛋白质的产生,而不是通过抑制翻译. 这一发现强调了mRNA破坏稳定是miRNA介导基因调节的主要机制.

更多相关视频

Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3'UTR-reporter Constructs and a microRNA Mimic in Adherent Cells
07:19

Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3'UTR-reporter Constructs and a microRNA Mimic in Adherent Cells

Published on: September 28, 2011

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs
11:00

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs

Published on: June 12, 2018

相关实验视频

Last Updated: Jun 10, 2026

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
08:40

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Published on: April 6, 2012

Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3'UTR-reporter Constructs and a microRNA Mimic in Adherent Cells
07:19

Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3'UTR-reporter Constructs and a microRNA Mimic in Adherent Cells

Published on: September 28, 2011

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs
11:00

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs

Published on: June 12, 2018

科学领域:

  • 分子生物学分子生物学
  • 遗传学 是一个遗传学.
  • 基因法规 基因法规

背景情况:

  • 微RNA (miRNA) 是小型非编码RNA,可以调节基因表达.
  • 通过转化抑制或mRNA降解,可以发生miRNA介导的基因抑制.
  • 这些机制的相对贡献,特别是对于内源性标,仍然在很大程度上没有特征.

研究的目的:

  • 量化转化抑制和mRNA降解对miRNA介导蛋白质生产减少的贡献.
  • 为了比较异位和内源miRNA目标的这些效应.
  • 阐明miRNAs调节蛋白质输出的主要机制.

主要方法:

  • 利用核糖体分析来测量全球蛋白质合成速率.
  • 同时评估信使RNA (mRNA) 水平的变化.
  • 分析了异位表达和内源调节的miRNA目标.

主要成果:

  • 减少的mRNA水平占了外阴和内生miRNA相互作用中蛋白质生产减少的大部分 (>=84%) .
  • 微RNA调节对蛋白质产量的影响与mRNA丰度的变化密切相关.
  • 确定mRNA不稳定是miRNA诱导蛋白质减少的主要驱动因素.

结论:

  • 通过miRNA介导的基因沉默主要通过目标mRNA的破坏稳定性而发生.
  • 在mRNA水平的变化作为miRNA调节影响的可靠指标.
  • 了解这种机制对于理解基因调节和开发基于miRNA的治疗方法至关重要.