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一个替代的拼接网络将细胞循环控制与细胞亡联系起来
Michael J Moore1, Qingqing Wang, Caleb J Kennedy
1Department of Systems Biology, Harvard Medical School, Boston, MA 02115, USA.
Cell
|August 14, 2010
概括
替代拼接调节细胞死亡,并在疾病中被破坏. 这项研究揭示了一种网络,通过关键基因的替代拼接,将细胞周期控制与细胞亡联系起来.
科学领域:
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
- 遗传学 是一个遗传学.
背景情况:
- 替代拼接是生物调节和多样性的关键机制.
- 替代拼接的失调与包括癌症在内的各种疾病有关.
- 细胞亡因子的Bcl2家族在编程细胞死亡中发挥着关键作用.
研究的目的:
- 研究人类细胞中替代拼接的全球控制.
- 为了确定Bcl-x和Mcl1mRNA的替代拼接的调节者.
- 通过剪接了解细胞循环控制和细胞亡之间的联系.
主要方法:
- 全基因组siRNA屏幕用于识别拼接调节器.
- 对Bcl2家族亡因子的mRNA拼接模式的分析.
- 研究细胞循环因子和极光激酶A.的作用.
主要成果:
- 一个细胞循环因子网络,包括极光激酶A,被确定为Bcl-x和Mcl1拼接的调节者.
- 由药物或siRNAs诱导的线粒体停止促进了Bcl-x,Mcl1和caspase-9.9的亲细胞分裂.
- 拼接调节器ASF/SF2的翻译后周转与拼接变体的变化相关,影响了亡.
结论:
- 一个新的替代拼接网络将细胞周期控制与细胞亡联系起来.
- 协同调节前死亡结合变体的上调促进细胞中亡的细胞经历了线索性停止.
- 拼接调节器ASF/SF2通过替代拼接在调节亡方面发挥着全球作用.
相关概念视频
Alternative RNA Splicing
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
The Cell Cycle Control System
The cell cycle regulation directs how a cell proceeds from one phase to the next and begins mitosis. The cell cycle control system includes intracellular regulatory molecules and external triggers. They provide "stop" or "advance" signals and operate at specific cell cycle stages termed checkpoints to ensure that a particular process is completed before the cell advances to the next phase.
Cyclins and cyclin-dependent kinases (Cdks) are the primary cell cycle regulators and function at the cell...
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The cell cycle is an organized set of events that leads the cell to divide into two daughter cells, each containing chromosomes identical to the parent cell. It is the cell cycle that leads to the formation of an entire organism from a single-cell zygote. Besides, cell division also functions in the renewal or repair of tissues in adult multicellular eukaryotes. For example, in the bone marrow, the stem cells divide to form new blood cells. Although essential for several functions, cell...

