哈斯宾的Histon H3 Thr-3酸化将Aurora B定位在线粒分裂中的中间体上
Fangwei Wang1, Jun Dai, John R Daum
1Division of Rheumatology, Immunology, and Allergy, Brigham and Women's Hospital, Harvard Medical School, Smith Building, 1 Jimmy Fund Way, Boston, MA 02115, USA.
Science (New York, N.Y.)
|August 14, 2010
概括
哈斯生成的素H3氨酸3酸化 (H3T3ph) 对于将染色体乘客复合体 (CPC) 招募到中心分子至关重要. 这种相互作用对于精确的染色体分离在分裂过程中至关重要.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 染色体乘客复合体 (CPC) 对于精确的染色体分离和细胞动力学是必不可少的.
- 负责将CPC招募到中间体的特定染色质因子仍未确定.
研究的目的:
- 为了研究基因组修饰在招募CPC到中间体中的作用.
- 为了识别介导CPC中心层定位的染色质因子.
主要方法:
- 研究了H3T3ph对于中心分子中CPC积累的必要性.
- 使用一个Survivin突变 (Survivin-D70A/D71A) 来评估与H3T3ph.的结合.
- 研究了Haspin枯竭和Survivin突变对MCAK的中心局部化和线粒检查点反应的影响.
- 评估了Survivin突变和H3T3ph特异性抗体微注射对中心极光B功能的影响.
主要成果:
- 哈斯宾对素H3氨酸3 (H3T3ph) 的酸化对于CPC的中心积累至关重要.
- CPC子单元Survivin直接与H3T3ph结合在一起.
- 一种非结合性的Survivin突变会损害中间体CPC度,MCAK局部化和线粒体检查点反应.
- 与Survivin突变体和H3T3ph抗体一起观察到受损的中心光线Aurora B功能,但细胞动力没有受到影响.
结论:
- 哈斯介导的H3T3ph作为CPC招募到中心分子的关键平台.
- 这种依赖H3T3ph的招募在线粒分裂过程中调节了特定的中心极光B功能.
- 鉴定的机制突出显示了一种确保精确染色体分离的新途径.
相关概念视频
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02:27The Mitotic Spindle
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