单聚烯复合体交叉链接动态元件,以调节染色体-微管附着的染色体
Kevin D Corbett1, Calvin K Yip, Ly-Sha Ee
1Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 250 Longwood Avenue, Boston, MA 02115, USA.
Cell
|August 21, 2010
概括
Csm1/Lrs4单聚复合体充当分子,确保细胞分裂期间适当的染色体附着,并保持DNA沉默. 它的V形结构是酵母中这些功能的关键.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 单聚合物复合物调节真菌中的动态 - 微管附着物.
- 它确保了Saccharomyces cerevisiae meiosis I.中的姐妹染色体的共同方向.
- 它抑制了schizosaccharomyces pombe 中的甲状腺附着,并保持了核糖体DNA (rDNA) 沉默.
研究的目的:
- 为了阐明S. cerevisiae Csm1/Lrs4 monopolin亚复合体的结构和功能.
- 为了研究Csm1在kinetochore-microtubule附着和rDNA沉默中的作用.
主要方法:
- 对Csm1/Lrs4子综合体的结构分析.
- Csm1的局部定向突变发生,以破坏蛋白质与蛋白质之间的相互作用.
- 姐妹染色体共导向在半变化I.的分析.
- 对rDNA沉默的评估.
主要成果:
- 细菌S. cerevisiae Csm1/Lrs4亚复合体呈现出一个V形结构,相互作用域间距离约为10nm.
- Csm1通过一个保存的疏水性表面斑块结合了kinetochore蛋白Dsn1和Mif2/CENP-C. Csm1通过一个保存的疏水性表面斑块结合了kinetochore蛋白Dsn1和Mif2/CENP-C.
- 破坏这些相互作用的Csm1点突变会损害姐妹染色体的共同定向和rDNA沉默.
- 突变可能会破坏与rDNA相关蛋白Tof2.2的结合.
结论:
- Csm1/Lrs4 作为一个分子,交叉连接基因基因组件,以确保适当的染色体分离.
- 这种紧机制抑制了甲状腺的附着,并强制执行同定向.
- Csm1/Lrs4还交叉链接rDNA重复,有助于rDNA沉默.
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