通过Cdk1对CPC的酸化促进了染色体双导向
Tatsuya Tsukahara1, Yuji Tanno, Yoshinori Watanabe
1Laboratory of Chromosome Dynamics, Institute of Molecular and Cellular Biosciences, University of Tokyo, Yayoi, Tokyo 113-0032, Japan.
Nature
|August 27, 2010
概括
在细胞分裂过程中,适当的染色体分离依赖于正确的螺旋附着. 这项研究揭示了Survivin的Cdk1-cyclin B酸化如何将染色体乘客综合体 (CPC) 向中位素,确保精确的染色体双方向性并防止不稳定性.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 细胞分裂期间精确的染色体分离对于基因组稳定性至关重要.
- 染色体双向的缺陷会导致染色体的不稳定性和癌症的进展.
- 染色体乘客综合体 (CPC) 调节染色体螺旋附着物,但其中位粒向性知之甚少.
研究的目的:
- 阐明调节CPC的中粒体向的保守机制.
- 为了确定关键的因素,参与确保染色体在线分裂期间的双导向.
- 研究Cdk1-cyclin B在CPC局部化和功能中的作用.
主要方法:
- 一种裂变酵母环林B突变体的分离和表征.
- 在Survivin.上确定Cdk1-cyclin B-依赖的酸化部位.
- 在野生类型和突变细胞中分析CPC向和染色体双导向.
- 在人类细胞中研究Survivin-shugoshin相互作用和玻利林酸化.
主要成果:
- 发现了一种新型的裂变酵母环林B突变,该突变在染色体双导向方面具有特殊缺陷.
- 对Survivin的Cdk1-环素B依赖酸化对于CPC中位素向和染色体双导向至关重要.
- 相仿生者拯救了B型旋突变的双方向缺陷.
- 幸存者的酸化促进了与CPC适配器舒戈辛的结合,北素酸化在人体细胞中发挥了类似的作用.
结论:
- 这项研究揭示了CPC向中间体的保守分子机制.
- Cdk1-cyclin B-dependent 幸存者酸化是染色体双导向的关键调节者.
- 舒戈辛 (Shugoshin) 作为CPC的保存中心介质适配器,将酸化事件与复杂的局部化联系起来.
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