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Updated: May 30, 2026

In vitro Transcription and Capping of Gaussia Luciferase mRNA Followed by HeLa Cell Transfection
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In vitro Transcription and Capping of Gaussia Luciferase mRNA Followed by HeLa Cell Transfection

Published on: March 26, 2012

确定mRNA 5'-end capping的质量控制机制

Xinfu Jiao1, Song Xiang, Chanseok Oh

  • 1Department of Cell Biology and Neuroscience, Rutgers University, Piscataway, New Jersey 08854, USA.

Nature
|August 31, 2010
PubMed
概括

酵母Rai1蛋白从信使RNA中去除异常的5'-end盖结构,揭示了mRNA完整性的质量控制机制. 这一过程确保了适当的mRNA封闭和稳定性.

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In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...
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In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
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