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来自火 luciferase 的循环 alkylaminoluciferin 基底的强烈光辐射

Gadarla Randheer Reddy1, Walter C Thompson, Stephen C Miller

  • 1Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 364 Plantation Street, Worcester, Massachusetts 01605, USA.

Journal of the American Chemical Society
|September 11, 2010
PubMed
概括

新的合成循环氨酸化素通过Ultra-Glo luciferase提供了增强的红移光辐射. 这些新型基板提供优越的光输出,与本地D-露西费林和其他类似物相比.

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Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...