在含有侧链组的β-中12螺旋二次结构的晶体学表征
Soo Hyuk Choi1, Ilia A Guzei, Lara C Spencer
1Department of Chemistry, University of Wisconsin, Madison, Wisconsin 53706, USA.
Journal of the American Chemical Society
|September 11, 2010
概括
这项研究报告了12个螺旋式β-的5个新晶体结构,揭示了详细的形状参数. 这些发现提高了对β-折叠和各种氨基酸残留在螺旋结构中的容纳的理解.
科学领域:
- 生物化学和结构生物学.
- 化工和折叠分子研究.
- 超分子化学和自我组装
背景情况:
- β-是一种多功能折叠体,具有多样化的二次结构,包括螺旋体.
- 这12个螺旋体的特征是i,i+3个脊柱键,模仿α-螺旋体.
- 了解12螺旋结构对于设计具有特定空间布局的功能β-来说至关重要.
研究的目的:
- 阐明包含多种侧链的12螺旋β-的详细固态结构.
- 为了描述12个螺旋折的形状参数.
- 为了研究12螺旋脚手架内修改的氨基酸残留物的兼容性.
主要方法:
- 采用X射线晶体学,确定了五种新型12螺旋β-的三维结构.
- 对晶体结构的分析,以得出平均骨干扭转角度和螺旋参数.
- 结构数据与关于β-和α-螺旋体的现有知识进行比较.
主要成果:
- 确定了五个全折叠的12螺旋β-的新晶体结构.
- 结构包括转-4,4-二甲基-2-氨基cyclopentanecarboxylic 酸 (dm-ACPC) 的残留物和β(3) -同氨酸 (β(3) -hPhe) 的残留物.
- 衍生的结构参数与其他i,i+3结合螺旋相比,证实了12螺旋的稳定性.
- 证明了12-螺旋体内β(3)-残留物的容纳.
结论:
- 12螺旋形状坚固,可以容纳各种侧链和修改后的残留物.
- 结构数据为功能β-的合理设计提供了基础.
- 这些发现有助于更深入地了解折叠机形状的多样性和稳定性.
相关概念视频
01:22Protein Folding
Overview
01:25Protein Folding
Proteins are chains of amino acids linked together by peptide bonds. Upon synthesis, a protein folds into a three-dimensional conformation, critical to its biological function. Interactions between its constituent amino acids guide protein folding, and hence the protein structure is primarily dependent on its amino acid sequence.
Protein Structure Is Critical to Its Biological Function
Proteins perform a wide range of biological functions such as catalyzing chemical reactions, providing...
Protein Structure Is Critical to Its Biological Function
Proteins perform a wide range of biological functions such as catalyzing chemical reactions, providing...
01:13Protein Organization
Overview
01:24Protein Organization
Proteins are polymers of amino acid residues. They are versatile and responsible for different cellular functions, including DNA replication, molecular transport, catalysis, and structural support. Proteins have a hierarchical structure comprising at least three levels of organization: primary, secondary, and tertiary structure. Some large proteins have a quaternary structure where individual protein subunits are linked together.
The primary structure of a protein is its amino acid sequence.
The primary structure of a protein is its amino acid sequence.
02:15Protein and Protein Structure
Proteins are one of the most abundant organic molecules in living systems and have the most diverse range of functions of all macromolecules. Proteins may be structural, regulatory, contractile, or protective. They may serve in transport, storage, or membranes; or they may be toxins or enzymes. Their structures, like their functions, vary greatly. They are all, however, amino acid polymers arranged in a linear sequence.
A protein's shape is critical to its function. For example, an enzyme can...
A protein's shape is critical to its function. For example, an enzyme can...
02:43Peptide Bonds
A peptide bond covalently attaches amino acids through a dehydration reaction. One amino acid's carboxyl group and another amino acid's amino group combine, releasing a water molecule. The resulting bond is the peptide bond. The products that such linkages form are peptides. As more amino acids join this growing chain, the resulting chain is a polypeptide. Each polypeptide has a free amino group at one end. This end has the N-terminal, or the amino-terminal, and the other end has a free...