诺奇和EGFR通路的相互作用调节神经干细胞数量和自我更新
Adan Aguirre1, Maria E Rubio, Vittorio Gallo
1Center for Neuroscience Research, Children's National Medical Center, Washington, District of Columbia 20010, USA.
Nature
|September 17, 2010
概括
大人的大脑中神经干细胞 (NSC) 和神经前代细胞 (NPC) 的平衡.
科学领域:
- 神经科学是一个神经科学.
- 干细胞生物学 干细胞生物学
- 细胞信号传递 细胞信号传递
背景情况:
- 细胞微环境或,调节干细胞的行为,包括自我更新和命运.
- 成年大脑的腹腔下区域 (SVZ) 含有神经干细胞 (NSC) 和神经前代细胞 (NPC).
- 在SVZ中维持NSC和NPC之间的平衡对于大脑功能和修复至关重要.
研究的目的:
- 研究表皮生长因子受体 (EGFR) 和Notch信号通路之间的相互作用,以维持SVZ内的NSC和NPC种群.
- 阐明EGFR和Notch信号相互作用调节成年大脑中神经干细胞和祖细胞平衡的机制.
主要方法:
- 使用成年SVZ利基模型的体内研究.
- 操纵EGFR信号,以评估其对NSC和NPC种群的影响.
- 细胞与细胞相互作用和信号通路交叉的分析 (EGFR和Notch).
主要成果:
- 在体内增强的EGFR信号导致NPC池的扩大.
- 增加EGFR信号减少了NSC的数量,并阻碍了NSC的自我更新.
- 这种效应是由一个非细胞自主机制介导的,EGFR信号调节了Notch信号.
结论:
- 在成年SVZ中发现了EGFR和Notch信号通路之间的新型相互作用.
- 这种交叉连接对于维持神经干细胞和祖细胞之间的平衡至关重要.
- 这些发现为成人大脑中NSC和NPC池维护的调节提供了一个关键机制.
相关概念视频
03:14Notch Signaling Pathway
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
03:14Notch Signaling Pathway
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
01:12Role Of Notch Signalling In Intestinal Stem Cell Renewal
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
01:26Stem Cell Niche
The stem cell niche is the dynamic microenvironment where stem cells reside. Inside these niches, the cells may remain undifferentiated, undergo high self-renewal, or become lineage-specific progenitors. Stem cells coexist with other niche cells, such as stromal cells. They also interact closely with the ECM. Cell-cell and cell-matrix communication occur via adhesion molecules or soluble factors that signal the stem cells and determine their fate. Stromal cells also provide survival signals to...
01:19Multipotency of Hematopoietic Stem Cells
The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...
01:12Renewal of Skin Epidermal Stem Cells
The skin is divided into epidermis, dermis, and hypodermis, the skin's outermost, middle, and inner layers. The human epidermal layer regularly undergoes renewal, where old, dead cells are replaced by new cells. Epidermal stem cells or EpiSCs divide and differentiate to restore the lost cells. For the renewal process, some EpiSCs continuously self-renew. In contrast, few others differentiate into transit-amplifying cells, which later form prickle or spinous cells, followed by granular cells,...