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Precise, High-throughput Analysis of Bacterial Growth
Published on: September 19, 2017
由格式驱动的增长加上H(2) 产量增长.
Yun Jae Kim1, Hyun Sook Lee, Eun Sook Kim
1Korea Ocean Research & Development Institute, PO Box 29, Ansan 425-600, Korea.
Nature
|September 17, 2010
概括
一些高热友的古生物只能使用酸盐作为能量来源生长,产生. 这种形式的新陈代谢对单个菌株来说在能量上是有利的,与之前观察到的合成菌群不同.
科学领域:
- 微生物学 微生物学
- 生物化学 生物化学
- 考古物 (Archaea) 是一种古老的物种.
背景情况:
- 甲酸盐转化为二碳酸盐和二氧化 (ΔG° = +1.3 kJ mol−1) 之前被认为是微生物生长的能量不足.
- 之前的研究表明,合成微生物群落的形式驱动的增长,依赖于甲基生物的消耗,使反应变得有力的.
研究的目的:
- 为了研究一个微生物是否可以使用酸盐作为唯一的能量来源生长,催化其转化为二碳酸盐和.
- 阐明超热友古生物体中形成代谢和能量生成的基础生物化学机制.
主要方法:
- 培养超热友的古生物,特别是热球菌 (Thermococcus onnurineus) 菌株NA1,以酸盐作为唯一的能量来源.
- 在生理生长条件下测量吉布斯自由能量 (ΔG) 变化.
- 在形式存在的情况下检测ATP合成.
- 基因表达造型和基因破坏实验,以确定参与格式代谢的关键基因.
主要成果:
- 一些超热友的古生物,包括Thermococcus onnurineus菌株NA1,表现出通过氧化酸盐和产生H的生长能力.
- 甲酸盐代谢计算的实际 ΔG 值在 -8 到 -20 kJ mol-1 之间,这表明在生长条件下发生了强力运动过程.
- 当甲酸盐是唯一的能量来源时,ATP合成得到了确认.
- 编码格式酶,阴离子/质子反载体和格式载体的特定基因集群被确定为格式驱动增长的关键.
结论:
- 这项研究为单个微生物的形式驱动生长提供了第一个证据,利用质子作为电子接受器.
- 鉴定出来的基因集群为Thermococcus onnurineus NA1.1中这种独特的代谢能力提供了生化基础.
- 这一发现扩大了我们对微生物能量代谢和在能源有限的环境中生命的潜力的理解.
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