一种反应敏捷的欧(III) 酸盐,可通过MRI直接读取pH值
Yunkou Wu1, Todd C Soesbe, Garry E Kiefer
1Department of Chemistry, University of Texas at Dallas, P.O. Box 830668, Richardson, Texas 75083, USA.
Journal of the American Chemical Society
|September 22, 2010
概括
一种新型的欧 (III) 复合物可以通过MRI进行pH成像. 这种化学交换和转移 (CEST) 剂通过监测明显的水交换峰值转移来检测pH值的变化,提供直接的pH值读取.
科学领域:
- 医疗成像医学成像
- 化学生物学 化学生物学
- 纳米技术纳米技术
背景情况:
- 精确的pH监测对于诊断和理解各种生理和病理过程至关重要.
- 目前基于MRI的pH成像方法通常需要度校准或受到有限的灵敏度的影响.
- 开发用于基于MRI的pH传感的响应性对比剂仍然是活跃的研究领域.
研究的目的:
- 开发和描述一种新的欧 (III) DO3A-tris (胺) 复合物,用于使用MRI进行pH成像.
- 调查化学交换和转移 (CEST) 原则的潜力,用于敏感的pH检测.
- 建立一种度独立的方法,用于在溶液中直接读取pH值.
主要方法:
- 一个欧 (III) DO3A-tris (胺) 复合物的合成和特征.
- 使用了比例化学交换和转移 (CEST) 的MRI原理.
- 采集了两种不同的激活频率的CEST图像,以实现直接的pH量化.
主要成果:
- 欧洲 (III) 复合体表现出pH取决于pH的CEST峰值在pH6和7.6.6之间变化.
- 一个性质子的脱质化导致了可观测的光谱转移,大约为5 ppm.
- 分量测量CEST方法提供了直接和度独立的溶液pH值读数.
结论:
- 开发的欧 (III) DO3A-tris (胺) 复合物是基于MRI的pH成像的一个有前途的药物.
- 电比测量CEST成像为pH量化提供了一种敏感且直接的方法,不需要度标记.
- 这种方法在推进体内pH值监测应用方面具有重大潜力.
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