Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Nuclear Localization Signals and Import01:46

Nuclear Localization Signals and Import

6.2K
Proteins targeted to the nucleus carry short stretches of amino acid sequences called the nuclear localization signal or NLS. Classical nuclear localization signals are of two types: monopartite and bipartite NLS. Monopartite classical NLS (cNLS) consists of a single cluster of 4-8 amino acids. Bipartite cNLS consists of two clusters of  2-3 amino acids and a 9-12 residue long proline-rich linker bridging the two clusters. Signal clusters are rich in positively charged amino acids such as...
6.2K
Assembly of Signaling Complexes01:30

Assembly of Signaling Complexes

4.7K
Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
4.7K
Amplifying Signals via Second Messengers01:15

Amplifying Signals via Second Messengers

6.1K
Many receptor binding ligands are hydrophilic; they do not cross the cell membrane but bind to cell-surface receptors. Thus, their message must be relayed by second messengers present in the cell cytoplasm. There are several second messenger pathways, each with its own way of relaying information. For example, the G protein-coupled receptors can activate both phosphoinositol and cyclic AMP (cAMP) second messenger pathways. The phosphoinositol pathway is active when the receptor induces...
6.1K
Intracellular Signaling Affects Focal Adhesions01:17

Intracellular Signaling Affects Focal Adhesions

2.8K
Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
2.8K
Anchoring Junctions01:03

Anchoring Junctions

4.3K
Anchoring junctions are multiprotein complexes that help cells connect to other cells and the extracellular matrix. Anchoring junctions are present on the lateral and basal surfaces of cells, providing strong and flexible connections. Focal adhesions are often formed due to cell interactions with the ECM substrata, which initiate signal transduction via kinase cascades and other mechanisms. Together, they provide stability and tissue integrity. There are three types of anchoring junctions:...
4.3K
Selectins01:25

Selectins

3.6K
Cell adhesion is  an essential aspect of multicellularity. While stable cell interactions usually occur between cells of the same type, transient cell interactions occur between cells of different tissue types, such as between neutrophils and endothelial cells. Selectins are one class of cell adhesion molecules (CAMs) that bind carbohydrate ligands to form transient cell adhesion. They are rod-like proteins with a long extracellular part of variable length ending with the lectin domain,...
3.6K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Immunopathophysiology of immune checkpoint inhibitor-related pneumonitis: insights from multi-omics and immune profiling.

Immunological medicine·2026
Same author

Real-world effectiveness, safety and patient-reported outcomes of sarilumab in patients with rheumatoid arthritis: a 52-week, prospective, open-label, non-interventional, observational, nationwide, large-cohort study in Japan (PROFILE-J).

RMD open·2026
Same author

Lenvatinib Combined with PD-1 Blockade Therapy Benefits Gastric Cancers through Immunosuppressive Macrophage Modulation.

Cancer immunology research·2026
Same author

Distinct fibrosis-associated macrophage subsets coordinate iron metabolism in pulmonary fibrosis.

International immunology·2026
Same author

Impact of pre-existing interstitial lung abnormalities on pneumonitis risk and survival in chemo-naive patients with non-small-cell lung cancer receiving pembrolizumab plus chemotherapy.

Respiratory medicine·2026
Same author

Subset binding enables detection of multimodal patient subgroup patterns and drug target discovery in idiopathic pulmonary fibrosis.

Briefings in bioinformatics·2026

相关实验视频

Updated: May 3, 2026

Proteomics to Identify Proteins Interacting with P2X2 Ligand-Gated Cation Channels
16:36

Proteomics to Identify Proteins Interacting with P2X2 Ligand-Gated Cation Channels

Published on: May 19, 2009

14.0K

通过plexin受体传递semaphorin信号的结构基础.

Terukazu Nogi1, Norihisa Yasui, Emiko Mihara

  • 1Laboratory of Protein Synthesis and Expression, Institute for Protein Research, Osaka University, 3-2 Yamadaoka, Suita, Osaka 565-0871, Japan.

Nature
|October 1, 2010
PubMed
概括

半氨基复合体信号传递涉及从同位体转变为异位体的结构转换,揭示了细胞表面受体激活如何将信号传输到细胞内部.

更多相关视频

Use of pHluorin to Assess the Dynamics of Axon Guidance Receptors in Cell Culture and in the Chick Embryo
10:30

Use of pHluorin to Assess the Dynamics of Axon Guidance Receptors in Cell Culture and in the Chick Embryo

Published on: January 12, 2014

7.9K
A Pipeline to Investigate the Structures and Signaling Pathways of Sphingosine 1-Phosphate Receptors
12:27

A Pipeline to Investigate the Structures and Signaling Pathways of Sphingosine 1-Phosphate Receptors

Published on: June 8, 2022

3.1K

相关实验视频

Last Updated: May 3, 2026

Proteomics to Identify Proteins Interacting with P2X2 Ligand-Gated Cation Channels
16:36

Proteomics to Identify Proteins Interacting with P2X2 Ligand-Gated Cation Channels

Published on: May 19, 2009

14.0K
Use of pHluorin to Assess the Dynamics of Axon Guidance Receptors in Cell Culture and in the Chick Embryo
10:30

Use of pHluorin to Assess the Dynamics of Axon Guidance Receptors in Cell Culture and in the Chick Embryo

Published on: January 12, 2014

7.9K
A Pipeline to Investigate the Structures and Signaling Pathways of Sphingosine 1-Phosphate Receptors
12:27

A Pipeline to Investigate the Structures and Signaling Pathways of Sphingosine 1-Phosphate Receptors

Published on: June 8, 2022

3.1K

科学领域:

  • 分子生物学分子生物学
  • 细胞信号传递 细胞信号传递
  • 结构生物学 结构生物学

背景情况:

  • 赛马福林和plexin是关键的细胞信号分子,参与各种生物过程和人类疾病.
  • 体与plexin受体结合激活了细胞内GTPase激活蛋白 (GAP) 的活性,但结构机制尚不清楚.

研究的目的:

  • 为了阐明半氨酸复合素受体激活的结构机制.
  • 为了确定在结合前和结合后状态下,semaphorin 6A (Sema6A) 和plexin A2 (PlxnA2) 的结构

主要方法:

  • 使用X射线晶体学来确定Sema6A和PlxnA2碎片的结构.
  • 分析了Sema6A-PlxnA2的复杂结构.
  • 用突变的配体/受体进行了基于细胞的活性测定.

主要成果:

  • 在结合之前,Sema6A形成了一个"面对面"同极体,而PlxnA2采用了一个"头对面"同极体排列.
  • 赛马6A-PlxnA2复合物形成了一个2:2异构四聚合物,PlxnA2通过分离和对接到赛马6A同质聚合物而经历"伴侣交换".
  • 塞马6A的"面对面"同分体排列在生理上是相关的,并且在信号发送过程中保持.

结论:

  • 普莱克辛同极体-异极体转换是信号传输的关键.
  • 这些转换重新定位了plexin的分子轴,促进了信号传输到细胞质区域,并诱导了GAP域激活.