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相关概念视频

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...
Non-LTR Retrotransposons03:18

Non-LTR Retrotransposons

As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
Types of RNA01:20

Types of RNA

Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
Types of RNA01:23

Types of RNA

Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Inheritance of Chromatin Structures03:17

Inheritance of Chromatin Structures

Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying DNA...

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相关实验视频

Updated: Jun 8, 2026

A Computational Pipeline for Intergenic/Intragenic Enhancer RNA Quantification in Mouse Embryonic Stem Cells
06:02

A Computational Pipeline for Intergenic/Intragenic Enhancer RNA Quantification in Mouse Embryonic Stem Cells

Published on: October 28, 2025

在人体细胞中具有增强剂类功能的长非编码RNA.

Ulf Andersson Ørom1, Thomas Derrien, Malte Beringer

  • 1The Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA.

Cell
|October 5, 2010
PubMed
概括
此摘要是机器生成的。

长非编码RNAs (ncRNAs) 惊人地激活基因表达,挑战了他们以前对其功能的理解. 这项研究揭示了它们在调节关键发育基因中的作用.

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Optimized Quantitative Assessment of Enhancer RNA Stability in Mouse Embryonic Stem Cells
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相关实验视频

Last Updated: Jun 8, 2026

A Computational Pipeline for Intergenic/Intragenic Enhancer RNA Quantification in Mouse Embryonic Stem Cells
06:02

A Computational Pipeline for Intergenic/Intragenic Enhancer RNA Quantification in Mouse Embryonic Stem Cells

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科学领域:

  • 基因组学就是基因组学.
  • 分子生物学分子生物学
  • 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

  • 长非编码RNAs (ncRNAs) 代表了哺乳动物转录组的很大一部分,但它们的功能仍然在很大程度上是未知的.
  • 现有的研究主要将ncRNA与基因沉默机制联系起来,例如X失活和印记.

研究的目的:

  • 使用GENCODE人类基因组注释来表征长ncRNA.
  • 研究长ncRNAs在基因表达调节中的功能性作用.

主要方法:

  • 利用GENCODE注释来识别超过一千个长的ncRNA,在多个人类细胞系中表达.
  • 进行了基因枯竭实验,以评估对邻近蛋白质编码基因表达的影响.
  • 采用异质转录试验来确认ncRNAs在基因激活中的作用.

主要成果:

  • 鉴定了人类细胞系中长ncRNAs的一个子集的意想不到的增强器类功能.
  • 特定ncRNAs的枯竭导致附近蛋白质编码基因的表达减少,包括关键的发育调节者,如SCL (TAL1),Snai1和Snai2.
  • 证明了这些ncRNAs对于激活基因表达的必要性.

结论:

  • 揭示了长非编码RNA作为基因表达的激活剂的新型作用.
  • 突出了ncRNAs参与调节发展和分化所必需的关键基因的作用.
  • 挑战了ncRNAs仅仅作为基因沉默器的主导观点.