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相关概念视频

The Extracellular Matrix01:42

The Extracellular Matrix

In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.Composition of the Extracellular MatrixThe extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse molecules.
The Extracellular Matrix01:29

The Extracellular Matrix

Overview
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.
Composition of the Extracellular Matrix
The extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse...
Cell-matrix's Response to Mechanical Forces01:13

Cell-matrix's Response to Mechanical Forces

In animal cells, the extracellular matrix allows cells within tissues to withstand external stresses and transmits signals from the outside of the cell to the inside. The extracellular matrix is extensive, and its composition varies between different types of tissues. For example, the reticular fibers and ground substance make up the ECM in loose connective tissue, while collagen and bone minerals make up the ECM of bone tissue. 
Anchoring junctions mechanically attach a cell to the...
Extracellular Matrix01:26

Extracellular Matrix

Unlike epithelial tissue, which is composed of cells closely packed with little or no extracellular space in between, connective tissue cells are dispersed in a matrix. This extracellular matrix (ECM) is composed of fibrous proteins like collagen, elastin, and fibronectin in a ground substance consisting of interstitial fluid, cell adhesion proteins, and proteoglycans. The proteoglycans form a gel-like material in the spaces between cells and provide hydration, buffering, binding, and force...
Overview of Cell-Matrix Interactions01:24

Overview of Cell-Matrix Interactions

The extracellular matrix or ECM holds cells together to form a tissue and allows the cells within the tissue to communicate. ECM comprises proteins such as fibronectin, collagen, laminin, etc. The most abundant protein in this space is collagen. Collagen fibers are interwoven with carbohydrate-containing protein molecules called proteoglycans. ECM allows cell migration and provides a structural scaffold at cell adhesion that anchors the cell when the extracellular matrix proteins interact with...
Matrix Proteoglycans and Glycoproteins01:21

Matrix Proteoglycans and Glycoproteins

Proteoglycans are extensively glycosylated proteins, commonly found in the extracellular matrix, interwoven with collagen fibers. Hyaline cartilage, the most common type of cartilage in the body, consists of short and dispersed collagen fibers associated with large amounts of proteoglycans. These proteoglycans have long negative charges that attract cations, which in turn attract water molecules. This influx of ions and water molecules swells up the proteoglycan like a water-soaked gel that can...

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相关实验视频

Updated: Jun 8, 2026

Design and Construction of Artificial Extracellular Matrix (aECM) Proteins from Escherichia coli for Skin Tissue Engineering
10:30

Design and Construction of Artificial Extracellular Matrix (aECM) Proteins from Escherichia coli for Skin Tissue Engineering

Published on: June 11, 2015

一个结构调节的基于DNA的细胞外矩阵.

Faisal A Aldaye1, William T Senapedis, Pamela A Silver

  • 1Department of Systems Biology, Harvard Medical School, 200 Longwood Avenue, Boston, Massachusetts 02115, United States. faisal_aldaye@hms.harvard.edu

Journal of the American Chemical Society
|October 8, 2010
PubMed
概括
此摘要是机器生成的。

研究人员为细胞支架创建了新的DNA/蛋白质矩阵. 这些可编程生物材料精确地控制细胞的行为和结构,模仿自然的细胞外环境,用于研究应用.

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A Rapid, Scalable Method for the Isolation, Functional Study, and Analysis of Cell-derived Extracellular Matrix
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Preparation of Tunable Extracellular Matrix Microenvironments to Evaluate Schwann Cell Phenotype Specification
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相关实验视频

Last Updated: Jun 8, 2026

Design and Construction of Artificial Extracellular Matrix (aECM) Proteins from Escherichia coli for Skin Tissue Engineering
10:30

Design and Construction of Artificial Extracellular Matrix (aECM) Proteins from Escherichia coli for Skin Tissue Engineering

Published on: June 11, 2015

A Rapid, Scalable Method for the Isolation, Functional Study, and Analysis of Cell-derived Extracellular Matrix
09:40

A Rapid, Scalable Method for the Isolation, Functional Study, and Analysis of Cell-derived Extracellular Matrix

Published on: January 4, 2017

Preparation of Tunable Extracellular Matrix Microenvironments to Evaluate Schwann Cell Phenotype Specification
07:50

Preparation of Tunable Extracellular Matrix Microenvironments to Evaluate Schwann Cell Phenotype Specification

Published on: June 2, 2020

科学领域:

  • 生物材料科学 生物材料科学
  • 分子工程分子工程分子工程
  • 细胞生物学 细胞生物学

背景情况:

  • 细胞外矩阵 (ECM) 对细胞行为和组织结构至关重要.
  • 当前的ECM模型往往缺乏精确的结构控制和可编程性.
  • 将DNA纳米技术与蛋白质工程相结合,为合成ECM提供了新的可能性.

研究的目的:

  • 通过结合DNA纳米技术和蛋白质工程来开发一种新型的人造细胞外矩阵.
  • 为了证明这些DNA/蛋白质矩阵对ex vivo细胞支架的有用性.
  • 建立一个模块化平台,用于创建可编程的ECM,模仿自然细胞.

主要方法:

  • 结合了DNA纳米技术原理与蛋白质工程技术.
  • 制造的DNA/蛋白质基矩阵用于细胞支架.
  • 设计单链DNA域调整矩阵持久度长度和刚度.
  • 在工程矩阵上培养人类子宫癌细胞.
  • 分析了细胞粘附,活力,迁移,细胞骨安排,p-FAK信号传递和FOXO1a转录因子局部化.

主要成果:

  • 人类宫癌细胞在DNA/蛋白质矩阵上表现出强大的粘附性,高活力和快速迁移.
  • 通过工程DNA域来实现DNA/蛋白质矩阵的结构调整性.
  • 矩阵刚度和持久度的长度影响了细胞细胞骨组织和形状.
  • 细胞信号传递 (p-FAK) 和转录因子局部化 (FOXO1a) 被工程矩阵属性调节.

结论:

  • 一个新的可编程类型,基于DNA/蛋白质的人造细胞外矩阵已经成功开发出来.
  • 这些矩阵为ex vivo细胞支架和细胞矩阵相互作用的研究提供了一个多功能平台.
  • 简单而模块化的结构允许系统地复制和研究自然的细胞外.