通过因诺西-酸盐增强的COI1-JAZ共受体对雅斯蒙酸盐的感知
Laura B Sheard1, Xu Tan, Haibin Mao
1Department of Pharmacology, Box 357280, University of Washington, Seattle, Washington 98195, USA.
植物激素称为jasmonates被一个复杂的COI1和JAZ蛋白质感知. 这一发现揭示了植物中莉花的感知和激素信号的分子机制.
科学领域:
- 植物生物学 植物生物学
- 分子植物科学 分子植物科学
- 激素信号传递 激素信号传递
背景情况:
- 斯酸盐是关键的植物激素,调节生长,发育和应激反应.
- 众所周知,F盒蛋白CORONATINE INSENSITIVE 1 (COI1) 通过准JAZ蛋白进行降解来调节斯蒙酸信号传递.
- 莉花的感知精确机制一直是难以捉摸的.
研究的目的:
- 为了阐明在Arabidopsis. jasmonate的感知分子机制.
- 为了识别斯酸盐受体复合物的组成部分.
- 描述激素识别和信号传递的结构基础.
主要方法:
- 结构生物学 (X射线晶体学)
- 药理学测定 药理学测定 药理学测定
- 蛋白质与蛋白质相互作用的生物化学分析.
主要成果:
- 阿拉比多普西斯的莉花酸受体是一个由COI1和JAZ蛋白质组成的多组件复合体.
- COI1 对生物活性激素 (3R,7S) -jasmonoyl-l-isoleucine (JA-Ile) 具有特定的结合口袋.
- 对于高亲缘激素结合和COI1相互作用来说,双部分JAZ降解子序列是必不可少的.
- 伊诺西 pentakisphosphate 作为第三个关键成分,桥接COI1和JAZ.
结论:
- 雅斯莫纳酸的感知是由一个涉及COI1,JAZ和伊诺西托尔塔基酸盐的三元复合体介导的.
- 这项研究揭示了特定JA-Ile识别和信号传导的结构基础.
- F-box 蛋白质可以作为适应性,多组分信号中心在植物激素通路中发挥作用.
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